两种方法制备的脑组织切片激光共聚焦显微镜成像效果的比较研究  

Comparison of Vibratome and Frozen Sections of Brain Tissue in the Quality and Image of Confocal Laser Scanning Microscopy

作　　者：李贵苹[1] 朱晓娜[2] 易静[1] 杭勤[1] 

机构地区：[1]上海交通大学医学院生物化学与分子细胞生物学系,上海200025 [2]上海交通大学医学院解剖学与组织胚胎学系,上海200025

出　　处：《中国细胞生物学学报》2015年第11期1528-1533,共6页Chinese Journal of Cell Biology

摘　　要：该文比较研究了振动切片法与冰冻切片法制作的脑组织切片的质量及其激光共聚焦显微镜成像的效果。振动切片法通过灌流固定、取小鼠脑、琼脂糖包埋后利用振动切片机连续冠状切片。冰冻切片法通过蔗糖脱水、OCT包埋液氮骤冷后利用冰冻切片机连续冠状切片。制备的脑组织切片进行免疫荧光染色,激光共聚焦显微镜观察成像效果。结果表明,与冰冻切片相比,振动切片法简单快速,且制片质量较高,脑组织切片无冰晶形成,抗原免遭破坏,组织结构较完整,更适用于脑组织样品的激光共聚焦显微镜的成像观察。We studied the quality of vibratome sections and frozen sections of brain tissues and evaluated the imaging quality of these two sections with laser scanning confocal microscope （LSCM）, respectively. Brain tissues of 6- to 8-week-old mice （CD 1） were fixed by perfusion and then embedded in low-melting agarose, sliced on a vibratome, or embedded in OCT compound, sliced on a freezing microtome. The vibratome sections and the frozen sections were demonstrated by immunofluorescence staining and observed by confocal laser scanning microscopy. The results showed that compared with frozen sections, vibratome sections of brain tissues offered distinct advantages： easier embedding approaches, higher quality of brain tissues sections, better structural integrity of tissue sections, and superior image quality of confocal laser scanning microscopy.

关 键 词：脑组织 振动切片 冰冻切片 激光共聚焦显微镜 

分 类 号：R361.2[医药卫生—病理学]