运用HPLC结合PCA技术对不同炮制程度的姜炭中5个姜辣素成分的含量比较研究  

HPLC combined with PCA technology for analysis of five gingerol compounds in different processing degrees of ginger charcoal

作　　者：于江泳[1] 陈求芳[2] 卢国勇[2] 

机构地区：[1]国家药典委员会,北京100061 [2]广东药学院中药学院,广东广州510006

出　　处：《中国中药杂志》2015年第21期4200-4204,共5页China Journal of Chinese Materia Medica

基　　金：国家中医药行业科研专项全国中药饮片炮制技术规范研究项目(201207004)

摘　　要：建立同时测定不同炮制程度姜炭中5个姜辣素成分的含量,并采用PCA主成分分析法进行比较分析。采用Ultimate TM XB-C18（4.6 mm×250 mm,5μm）色谱柱,流动相为乙腈（A）-0.1%磷酸水（B）,梯度洗脱,检测波长为280 nm,流速0.6 m L·min-1,柱温30℃。5个成分分离度良好,标准曲线在线性范围内相关性良好,r≥0.999 7。加样回收率均值在98.86%~101.5%,RSD 1.4%~2.9%。不同炮制程度姜炭所含姜辣素成分在组分和含量上均存在一定差异,姜酮在标炭中含量最高,姜辣素类成分随着炮制程度的加深,含量依次降低。PCA模式识别显示不同炮制程度的姜炭聚为3类,为建立姜炭的质量标准提供了科学依据。To establish a new method for simultaneously determining the content of five gingerol compounds in different processing degrees of ginger charcoal and PCA principal component analysis was conducted for analysis. Samples were analyzed on Ultimate TM XB-C18column（ 4. 6 mm × 250 mm,5 μm）,with acetonitrile（ A）-0. 1% phosphoric acid solution（ B） as mobile phase for gradient elution. Detection wavelength was set at 280 nm. The flow rate was 0. 6 m L·min- 1and the column temperature was 30 ℃. The five compounds were separated well and showed good linearity（ r≥0. 999 7） within the concentration ranges tested. The average value for recoveries was between 98. 86%-101. 5%（ RSD 1. 4%-2. 9%）. The contents of five compounds showed difference among different processing degrees of ginger charcoal. Zingiberone had the highest content in the standard carbon,and the content of gingerol was decreased as the deepening of processing degree. Different processing degrees of ginger charcoal were classified into three groups with PCA,and provided scientific basis for establishing the quality standards of ginger charcoal.

关 键 词：不同炮制程度姜炭 姜辣素 主成分分析 HPLC 

分 类 号：R283[医药卫生—中药学] O657.72[医药卫生—中医学]