阿魏酸、川芎嗪微透析脑探针回收率测定及影响因素研究  

Study on recovery and its influencing factors of ferulic acid and tetramethylpyrazine in cerebral microdialysis probe

作　　者：廖卫国[1] 王利胜[1] 范文涛[1] 李周[1] 余健烨 廖丰蕴[1] 吴银爱 巴文强[1] 王玎[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《中国中药杂志》2015年第21期4275-4280,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81373973);广东省科技计划项目(2013B032500015)

摘　　要：建立阿魏酸（FA）、川芎嗪（TMP）微透析探针的体内外回收率校正方法,考察影响回收率的因素,为进一步的体内微透析试验提供依据。运用高效液相色谱法测定微透析液中FA,TMP的含量,分别计算探针的回收率;利用浓度差法（增量法、减量法）分别考察流速、浓度、温度对FA,TMP微透析探针体外回收率的影响,并考察了探针体内回收率的稳定性。增量法和减量法在相同的条件下所测得的探针体外回收率近似相等;相同的条件下,FA,TMP探针体外回收率在流速为1~2.5μL·min-1均随着流速增加而降低,在25~42℃均随着温度上升而增加;而在同一流速下,探针体外回收率与FA,TMP浓度均无关;此外,FA,TMP的探针回收率在4个浓度循环中的变化基本保持稳定,且均呈现出相似的变化趋势,表明浓度差法的日内重复性良好。脑探针在大鼠体内8 h内的回收率可维持相对稳定,但不同的脑探针回收率之间存在一定的差异性,提示每次在体动物实验均需进行探针的回收率校正。微透析技术可用于FA,TMP的脑局部药代动力学研究,反透析法可作为体内同时研究FA,TMP探针回收率的测定方法。To establish a method for detecting microdialysis recovery of tetramethylpyrazine（ TMP） and ferulic acid（ FA） and investigating the influencing factors,providing the basis for further in vivo microdialysis experiments. The concentration of FA and TMP in dialysates were determined by high pressure liquid chromatography（ HPLC） and probe recovery were calculated respectively. The influence of the flow rates,medium concentration,temperature and in vivo probe stability on the recovery of FA and TMP were investigated by using concentration difference method（ incremental method and decrement method）. The recovery obtained by incremental method were similar to by decrement method. The in vitro recovery rate of FA and TMP decreased with the increase of 1-2. 5 μL min- 1,and increased obviously with the temperature of 25-42 ℃ under the same conditions. The concentration of FA and TMP had no obvious effect on the probe recovery under the same flow rate. In addition,the recovery of TMP and FA remained stable and showed similar trends under the condition of four concentration cycles,indicating that the intra day reproducibility of the concentration difference method was good. The recovery of brain microdialysis probes in vivo 8 h maintained a relatively stable,but certain differences existed between different brain microdialysis probes,demonstrating that each probe was required for recovery correction in vivo experiment. Microdialysis sampling can be used for the local brain pharmacokinetic study of FA and TMP,and retrodialysis method can be used in probe recovery of FA and TMP in vivo.

关 键 词：微透析 阿魏酸 川芎嗪 探针回收率 

分 类 号：R285.5[医药卫生—中药学]