同种异体骨髓间充质干细胞复合冻干骨修复下颌骨节段性缺损的实验研究  

An experimental study on segmental defects reconstruction of canine mandible with allogenic bone marrow mesenchymal stem cells combined with lyophilized bone

作　　者：王宏[1] 景彩霞[2] 谭新颖[1] 罗金超[1] 刘昌奎[1] 胡敏[1] 

机构地区：[1]解放军总医院口腔颌面外科,北京100853 [2]延安大学医学院病原生物学教研室

出　　处：《中华口腔医学杂志》2015年第12期720-724,共5页Chinese Journal of Stomatology

基　　金：国家自然科学基金（81470726）

摘　　要：目的 观察同种异体骨髓间充质干细胞（bone marrow mesenchymal stem cells,BMSC）复合冻干骨修复犬下颌骨节段性缺损的效果.方法 将36只比格犬分为3组,每组12只,分别为同种异体BMSC＋冻干骨修复组（A组）、同种自体BMSC＋冻干骨修复组（B组）、仅用冻干骨修复组（C组）.在36只比格犬下颌骨左侧制造30 mm节段性缺损.3组分别植入以上不同材料后4、12、24、48周处死动物,每个时间点3只,行CT及组织学检测.结果 CT检查,植入12周：A、B组冻干骨均有新骨形成,C组新骨形成不明显;植入48周：3个组下颌骨形态基本恢复,A、B组冻干骨的孔隙被新骨填充,C组冻干骨的孔隙仍较明显.组织学检测,植入12周：A、B组冻干骨与自体骨之间骨小梁大部分连接,大量纤维组织进入移植骨组织周围,有新骨形成;C组冻干骨吸收依然不明显,新骨形成明显较其他两组慢;植入48周：A、B组的冻干骨均被新骨替代,C组仅有少部分冻干骨被新骨替代.A和B组无明显差别.结论 同种异体BMSC与自体BMSC均能加速同种异体骨成骨,二者无明显差别.Objective To investigate the effect of segmental defects reconstruction of canine mandible with allogenenic bone marrow mesenchymal stem cells （BMSC） combined with lyophilized bone.Methods A 30 mm segmental defect was created on the left mandibles of beagles.Beagles were randomly divided into three groups.Allogeneic bone marrow mesenchymal stem cells with lyophilized bone were used for segmental defects reconstruction in group A.Autologous bone marrow mesenchymal stem cells with freeze-dried bone were used for segmental defects reconstruction in group B.The defects of group C were repaired with lyophilized bone only.Every three beagles were sacrificed 4, 12, 24, and 48 weeks after surgery respectively.The reconstruction effect was evaluated by CT and histopathological examination.Results CT examination showed that new bones formed in group A and group B 12 weeks after surgery, but not in group C.The form of the reconstructed mandibles in the three groups recovered in 48 weeks.The small pores on the bone graft were filled with new bones in group A and group B.In group C, the pores were still evident.Histopathological examination showed that bone trabecula between allogeneic bone and autogenous bone was completely joined in group A and group B.A large number of fibrous tissue appeared around the implanted bone and new bones were formed.In group C, the lyophilized bone resorption was still not obvious, the new bone formation was significantly slower than the other two groups.There was no difference between group A and group B.Conclusions Both allogeneic bone marrow mesenchymal stem cells and autologous mesenchymal stem cells could accelerate the bone formation.

关 键 词：骨髓祖代细胞 间质干细胞 下颌骨 修复重建 

分 类 号：R782.2[医药卫生—口腔医学]