多吡啶铜(Ⅱ)配合物的合成、表征与DNA作用及抗肿瘤活性  

Synthesis,Characterizes,Interaction with DNA and Antiproliferative Activity of Cancer Cells by Polypyridyl Copper(Ⅱ) Complex

作　　者：黄冉涛 李政道[1] 赵强[1] 摆杨 杨浩[1] 

机构地区：[1]南阳师范学院化学与制药工程学院,河南南阳473061

出　　处：《化学试剂》2015年第12期1080-1084,1090,共6页Chemical Reagents

基　　金：河南省科技厅基础与前沿技术研究资助项目(112300410189)

摘　　要：合成了一种多吡啶铜(Ⅱ)配合物,用红外光谱、元素分析、X-射线单晶衍射法对配合物的组成和结构进行了表征。该配合物的晶体属单斜晶系,P2(1)/c空间群。用荧光光谱、紫外光谱研究了配合物与小牛胸腺DNA的键合作用。结果表明,配合物与DNA的键合作用属部分插入,结合常数K_b=8.0×104L/mol。用凝胶电泳法研究了配合物对p BR322DNA的切割作用。实验表明,配合物用310 nm光辐射15 min,可将p BR322 DNA断裂为开口缺刻型和线型DNA。采用MTT法研究了配合物对人乳腺癌细胞和肺癌细胞的体外抗肿瘤活性。结果发现,所合成配合物能有效抑制这两种细胞增殖。A tridentate polypyridyl Cu（ Ⅱ） complex has been synthesized and confirmed by IR,elemental analysis,and X-ray single crystal diffraction. The crystal structure of complex belongs to monoclinic system with space group P2（ 1） / c. Interactions of the complex with calf thymus DNA binding have been investigated by fluorescence spectroscopy,absorption spectroscopy. The spectrophotometric results showed that the mode of binding could be partial intercalation and its binding constant Kb is 8. 0 × 104 L / mol.The agarose gel electrophoresis showed complex can cleave circular plasmid pBR322 DNA to both nicked and linear forms when it was irradiated at 310 nm for 15 min. In vitro cytotoxic activities of the complex against human breast carcinoma（ MCF-7） and human lung carcinoma（ A549） were investigated by MTT assay method. The results indicated that complex can effectively inhibit the proliferation.

关 键 词：多吡啶配体 铜(Ⅱ)配合物 晶体结构 DNA 键合 断裂 抗肿瘤活性 

分 类 号：O614[理学—无机化学]