浙贝母碱对A549/顺铂耐药肺癌细胞株核苷酸ERCC1基因及LRP表达的影响  

Effect of Peimine on ERCC1 mRNA and LRP Expressions of A549/DDP Multidrug Resistance Cell Line

作　　者：唐晓勇[1] 唐迎雪[2] 许鹏[3] 周海燕[1] 韩丽[4] 

机构地区：[1]山东省肿瘤医院内八科,济南250117 [2]山东中医药大学基础医学院,济南250355 [3]山东省肿瘤医院人力资源部,济南250117 [4]山东省肿瘤医院内七科,济南250117

出　　处：《中国中西医结合杂志》2015年第12期1490-1494,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：山东省中医管理局科研计划资助项目(No.2009-170);山东省自然基金-青年博士基金资助项目(No.BS2011YY052)

摘　　要：目的观察浙贝母碱对肺癌A549/顺铂(DDP)耐药细胞切除修复互补基因1(excision repair cross-complementation 1,ERCC1)及肺耐药蛋白(lung resistant protein,LRP)表达的影响。方法体外培养肺癌A549/DDP细胞,将对数生长期的细胞分为空白对照组、DDP组、川芎嗪组(DDP加川芎嗪)及浙贝母碱组(DDP加浙贝母碱)。药物作用48 h后,采用RT-PCR法检测ERCC1 mRNA表达,细胞免疫荧光法检测细胞LRP表达水平。结果 DDP组与空白对照组ERCC-1 mRNA及LRP蛋白表达水平比较,差异无统计学意义(P>0.05)。与DDP组比较,川芎嗪及浙贝母碱组ERCC1 mRNA及LRP蛋白表达水平明显降低(P<0.05);浙贝母碱组ERCC1 mRNA及LRP蛋白表达水平明显低于川芎嗪组(P<0.05)。结论浙贝母碱可逆转肺癌A549/DDP细胞株的多药耐药,其作用机制可能与抑制ERCC1 mRNA表达及LRP表达有关。Objective To explore the effect of peimine on excision repair cross-complementation 1（ ERCC1） mRNA and lung resistant protein（ LRP） expressions in A549 / cisplatin（ DDP） multidrug resistance（ MDR） cell line. Methods Lung cancer A549 / DDP cells were culturedin vitro. Cells at logarithmic growth phase were divided into 4 groups,i. e.,the blank control group,the DDP group,the ligustrazine group（ DDP ＋ ligustrazine）,the peimine group（ DDP ＋ peimine）.After 48-h drug action,ERCC1 mRNA expression was detected by RT-PCR and LRP expression detected by cell immunofluorescence. Results There was no statistical difference in expression levels of ERCC1 mRNA and LRP between the DDP group and the blank control group（P〈0. 05）. Compared with the DDP group,expression levels of ERCC1 mRNA and LRP obviously decreased in the ligustrazine group and the peimine group（P〈0. 05）. They were obviously lower in the peimine group than in the ligustrazine group（P〈0. 05）. Conclusions Peimine could reverse MDR of A549 / DDP cell line. Its mechanism might be associated with down-regulating ERCC1 mRNA and LRP expression levels.

关 键 词：浙贝母碱 肺癌 多药耐药 切除修复互补基因1 肺耐药蛋白 

分 类 号：R734.2[医药卫生—肿瘤]