不同海拔红松林土壤微生物功能多样性  

Soil microbial functional diversity of different altitude Pinus koraiensis forests

作　　者：韩冬雪[1] 王宁[1] 王楠楠[1] 孙雪[1] 冯富娟[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《应用生态学报》2015年第12期3649-3656,共8页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(41105104);长白山科学研究院开放基金项目(201506);中央高校基本科研业务费专项(2572015EA02)资助

摘　　要：为全面了解红松林土壤微生物碳源利用特点,以长白山海拔700-1100 m红松林0-5和5-10 cm表土为研究对象采用Biolog微平板法,分析了土壤微生物功能多样性沿海拔的垂直分布特征和变化规律.结果表明：不同海拔红松林土壤微生物功能多样性差异显著,平均每孔颜色变化率（AWCD）随培养时间延长而增加,同一深度土层的AWCD值随海拔升高而降低;Shannon、Simpson和McIntosh多样性指数也随海拔升高呈现下降趋势,且不同海拔间3个多样性指数差异显著;物种多样性和功能多样性表现出相同的变化规律.土壤微生物对6大类碳源利用强度存在差异各海拔土壤微生物对氨基酸类碳源利用率最高,为优势碳源;主成分分析表明不同海拔土壤微生物在碳源利用上有明显的空间分异,土壤微生物功能多样性垂直地带性差异主要体现在对碳水类、氨基酸类和羧酸类碳源的利用上,其中碳水类尤为突出.对不同海拔土壤微生物群落功能多样性聚类分析表明,样地植被组成会对土壤微生物组成和功能活性产生重要影响.In order to comprehensively understand the soil microbial carbon utilization characteristics of Pinus koraiensis forests,we took the topsoil（0-5 cm and 5-10 cm） along the 700-1100 m altitude in Changbai Mountains and analyzed the vertical distributed characteristics and variation of microbial functional diversity along the elevation gradient by Biolog microplate method.The results showed that there were significant differences in functional diversity of microbial communities at different elevations.AWCD increased with the extension of incubation time and AWCD at the same soil depth gradually decreased along with increasing altitude;Shannon,Simpson and McIntosh diversity index also showed the same trend with AWCD and three different diversity indices were significantly different along the elevation gradient;Species diversity and functional diversity showed the same variation.The utilization intensities of six categories carbon sources had differences while amino acids were constantly the most dominant carbon source.Principal component analysis（PCA） identified that soil microbial carbon utilization at different altitudes had obvious spatial differentiation,as reflected in the use of carbohydrates,amino acids and carboxylic acids.In addition,the cluster of the microbial diversity indexes and AWCD values of different altitudes showed that the composition of vegetation had a significant impact on soil microbial composition and functional activity.

关 键 词：红松林 海拔 土壤微生物 功能多样性 BIOLOG 

分 类 号：S714.3[农业科学—林学]