狭鳕皮胶原蛋白肽对过氧化氢诱导的人脐静脉内皮细胞氧化损伤的作用研究  

The effect of collagen peptide from Pollock skin against H_2O_2-induced injury in human umbilical vein endothelial cells

作　　者：满帅[1] 夏蕴秋[1] 仲伟珍[1] 王春波[1] 张德岩 苗德森 韩彦弢[1] 

机构地区：[1]青岛大学药学院,山东青岛266021 [2]青岛福生食品有限公司,山东青岛266300

出　　处：《中国海洋药物》2015年第6期65-68,共4页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(81473384);青岛市民生科技计划项目(14-2-3-50-nsh)资助

摘　　要：目的通过建立H_2O_2导致人脐静脉内皮细胞损伤的模型来研究狭鳕鱼皮胶原蛋白肽作用。方法用人脐静脉内皮细胞作为体外研究对象,采用不同的浓度(40、200、400μg/mL)胶原蛋白和维生素E预处理12h,然后在加入50μmol/L H_2O_2继续培养12h造成损伤模型。模型成功后用CCK-8检测吸光度,根据试剂盒操作检测细胞内及培养上清液谷胱甘肽过氧化物酶(GXH-PX),一氧化氮(NO)、过氧化氢酶(CAT),丙二醛(MDA)等指标的含量和活性变化。结果经胶原蛋白预保护后细胞增值率升高,GXH-PX、CAT等活性增强,NO含量升高,丙二醛含量降低。结论 50mmol/L H2O2能够成功复制人脐静脉内皮细胞损伤模型,胶原蛋白肽能够有效的对抗这种损伤作用且呈剂量依赖性。其保护作用机制可能与调控GXH-PX、CAT的活性以及NO、丙二醛的含量有关。Object To investigate the effect of collagen peptides on H_2O_2-induced injury in human umbilical vein endothelial cells（HUVEC）.Methods Cultured HUVEC in vitro was protected using different concentrations collagen peptides（40mol/L、200mol/L、400mol/L）and Vitamin E for 12 h,50mmol/L H_2O_2 cultured for another 12 h.The effect of proliferation of HUVEC was evaluated by CCK-8.Using HUVEC and cultured supernatant by KIT,the activity of GXH-PX and CAT,the content of NO and MDA were evaluated.Results Collagen peptides could promote the growth of HUVEC,the activities of GXH-PX and CAT were induced,the contents of NO increased and MDA decreased.Conclusions 50mmol/L H_2O_2 could induce the damage of HUVECs,collagen peptides could play an important role through its anti- oxidative effects,and the effects were related with its doses.This might be related with changing the activities of GXH-PX and CAT,the contents of NO and MDA.

关 键 词：胶原蛋白肽 内皮细胞 氧化损伤 

分 类 号：R965[医药卫生—药理学]