miR-421靶向抑制caspase-3表达调控BGC-823胃癌细胞的迁移与凋亡  

miR-421 regulates migration and apoptosis of human gastric cancer BGC-823 cells through caspase-3 signal pathway

作　　者：吴建红[1] 王泽友[2] 姚永良[1] 顾涛[1] 成阳[1] 王建军[1] 

机构地区：[1]江苏大学附属昆山医院检验科,江苏昆山215300 [2]中南大学肿瘤研究所,湖南长沙410078

出　　处：《实用肿瘤杂志》2015年第6期510-515,共6页Journal of Practical Oncology

基　　金：昆山市科技计划项目(KS1347)

摘　　要：目的探讨miR-421调控BGC-823胃癌细胞迁移及凋亡的分子机制。方法生物信息学方法分析miR-421的靶基因,Western blot分析miR-421靶标caspase-3蛋白在BGC-823细胞中的表达。Negative miRNA、miR-421 mimics及miR-421 inhibitors分别转染BGC-823细胞后模拟或抑制miR-421的表达,另设空白对照组。流式细胞仪检测BGC-823细胞经siRNA转染后的细胞凋亡情况,Transwell小室检测细胞转染后的迁移能力。结果miRNA靶基因预测软件获得7个重要的miR-421靶基因。Caspase-3蛋白在miR-421 inhibitors转染细胞中表达增强(P<0.05),miR-421 inhibitors转染的BGC-823细胞凋亡明显增多并且迁移能力受到明显抑制(均P<0.05)。结论 miR-421通过靶向作用于caspase-3基因影响caspase-3的信号通路,促进BGC-823胃癌细胞的迁移并抑制其凋亡。Objective To investigate the effect of miR-421 on the apoptosis and migration of human gastric cancer BGC-823 cells. Methods The potential miR-421 targeting genes were searched with bioinformatic methods and the targeting protein caspase-3 was identified by Western blot in BGC-823 cells. BGC-823 cells were treated with miR-421 mimics and inhibitors, respectively and the expression of caspase-3 was detected with Western blot. Cell apoptosis after transfection was evaluated with flow eytometry, and cell migration was determined with transwell assays. Results Seven target genes were obtained by the prediction software of miRNA targeting genes. The expression of caspase-3 was increased in BGC-823 cells transfected with miR-421 inhibitors, and the migration ability was inhibited and the apoptosis of BGC-823 cells was increased significantly （ all P 〈 0. 05 ）. Conclusion miR-421 can promote cell migration and inhibit apoptosis in human gastric cancer BGC-823 cells, in which caspase-3 signal pathway may be involved.

关 键 词：胃肿瘤/病理生理学 微RNAS 半胱氨酸天冬氨酸蛋白酶3 细胞运动 细胞凋亡 转染 

分 类 号：R735.2[医药卫生—肿瘤] R730.23[医药卫生—临床医学]