静注免疫球蛋白与盐酸伊立替康的相互作用  

Interaction of intravenous immunoglobulin and irinotecan hydrochloride

作　　者：刘永春[1] 齐慧丽[1] 魏晓霞[1] 姚小军[2] 李莹莹[1] 张克钧[1] 刘建宁[1] 

机构地区：[1]陇东学院化学化工学院,甘肃庆阳745000 [2]兰州大学化学化工学院,甘肃兰州730000

出　　处：《化学研究与应用》2015年第12期1841-1848,共8页Chemical Research and Application

基　　金：甘肃省应用化学重点培育学科资金项目(GSACKS20130113)资助;陇东学院博士科研启动资金项目(XYBY1501)资助

摘　　要：利用荧光光谱、傅里叶变换红外(FT-IR)光谱、紫外-可见吸收光谱及分子对接方法在体外研究了静注免疫球蛋白(IVIG)与盐酸伊立替康的相互作用。研究发现,静注免疫球蛋白(IVIG)与盐酸伊立替康的相互作用力类型主要为静电作用力并辅以氢键,是一个自发的熵驱动、非特异性的、较弱的蛋白-药物作用,在289-310 K和pH 4.0条件下其亲和力常数为1.167~1.790×104L·mol^(-1)(n=4)。药物加入前后IVIG的二级结构含量通过酰胺I带FT-IR差谱、解卷积以及二阶导数谱进行计算,结果发现药物加入后IVIG典型的β结构去折叠化程度显著。IVIG可作为盐酸伊立替康的药物转运蛋白。The interaction of intravenous immunog/obulin（IVIG） with irinotecan hydrochloride was studied in vitro by fluorescence spectra,fourier transformation infrared（FT-IR） spectra,ultraviolet-visible absorption speetraand molecular docking. It was found that the binding of IVIG to irinotecan hydroehloride, involving typical electrostatic interaction coupled with hydrogen bonding formation, was a spontaneous enthalpy driven, non-spoeific and weak drug-protein interaction with the affinity constant Ko of 1. 167 -1. 790×10^4 L·mol^-1 at 289- 310K and pH 4. 0 （n = 4 ）. The contents of secondary structural composition of free IVIG and its ifinoteean hydrochloride complex were calculated by FT-IR difference spectra, self-deconvolution, second derivative resolution enhancement and the curve-fitting procedure of amide I band, respectively. The observed spectral changes indicated a marked unfolding of the typical β structure of protein. IVIG could serve as a transport protein（carrier） for irinoteean hydroehloride.

关 键 词：分子对接 静注免疫球蛋白(IVIG) 盐酸伊立替康 相互作用 

分 类 号：O657.3[理学—分析化学]