免标记法快速检测牛奶中卡那霉素的研究  

Rapid Detection of Kanamycin in Milk Using Label-free Method

作　　者：王承克[1] 陈丹[1] 陆峰[1] 张俊俊[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212032

出　　处：《分析测试学报》2015年第12期1434-1438,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金(21305032);中国博士后科研基金(2014M551522);江苏省博士后科研基金(1402073B)

摘　　要：发展了一种利用纳米金探针免标记法检测卡那霉素的方法。适配体能够使纳米金在NaCl溶液中保持一定的稳定性,且溶液呈红色,而当卡那霉素存在时,由于适配体与卡那霉素特异性的相互作用,使纳米金的稳定性降低,当NaCl存在时发生聚集,溶液变为蓝紫色,根据溶液在630 nm波长处吸光度值的改变,利用具有固定波长的酶标仪可实现卡那霉素的快速定量检测。检测线性范围为1～10 nmol/L,检出限为1nmol/L,检测过程可在5 min内完成。该方法成功用于牛奶中卡那霉素的检测,具有较好的选择性和较高的灵敏度,对食品中抗生素超标等食品安全问题的监控具有重要意义。A label-free method was developed for the detection of kanamycin using gold nanoparticles（ GNPs）. This method was based on the fact that aptamer can make GNPs maintain monodispersed in Na Cl solution and keep red color. After adding kanamycin into the solution,due to the specific interaction of aptamers with kanamycin,the stability of GNPs was reduced as the aptamer was firstly interacted with kanamycin and removed from GNPs,which induced GNPs aggregating in the presence of Na Cl,and the color of the solution was changed into blue or blue-violet,correspondingly. According to the absorbance change of the solution at 630 nm,the content of kanamycin could be rapidly detected using a simple microplate reader with fixed wavelength. The linear range of kanamycin was in the range of 1-10 nmol / L,with a detection limit of 1 nmol / L. The detection process could be accomplished in 5 min. This method could be used to detect kanamycin in milk samples,with advantages of good selectivity and high sensitivity,and is of great significance to detect the excessive antibiotics addition in food.

关 键 词：卡那霉素 纳米金 适配体 牛奶 

分 类 号：O657.3[理学—分析化学] TQ460.72[理学—化学]