胞裂蛋白9基因甲基化在老年人群结直肠癌筛查中的意义  

Value of methylated Septin 9 detection in screening colorectal cancer in elderly people

作　　者：丁清清[1] 张皓[1] 许海尘[1] 李晓林[1] 邵耘[1] 薛绮萍[1] 孙为豪[1] 

机构地区：[1]南京医科大学第一附属医院老年消化科,210029

出　　处：《中华老年医学杂志》2015年第12期1348-1350,共3页Chinese Journal of Geriatrics

基　　金：江苏省六大人才高峰资助项目（WSW-027）,江苏省高校自然科学研究资助项目（J3KJB320012）;江苏省卫生厅干保保健课题（BJ14003）

摘　　要：目的探讨胞裂蛋白（SEPT）9基因甲基化（mSEPT9）检测在老年人群中筛查结直肠癌的意义。方法将对象分为三组，老年结直肠癌组82例，老年结直肠息肉组80例以及老年健康对照组100例。应用实时定量PCR法检测入组患者的外周血及粪便中的mSEPT9，分析其与结直肠临床病理特征的关系，分析两种检测手段是否具有一致性。结果老年结直肠癌组血浆mSEPT9的阳性率73．2％，老年结直肠息肉组6．3％，老年健康对照组4．0％。结直肠癌组mSEPT9的阳性率高于其他两组（P〈0．01）。血浆mSEPT9在结直肠癌患者中的敏感度73．2％，特异度95．6％。在结直肠肿瘤最大径大于3cm，癌灶侵及全层、分化较差和临床分期较晚的患者中，血浆mSEPT9阳性率较高（P〈0．05）。同一患者粪便中提取的DNA，mSEPT9的检测结果与在血浆中测得的结果完全一致。结论老年结直肠癌组的mSEPT9阳性率明显高于其他两组，敏感性、特异性均较高，入组患者的血浆和粪便的表达完全一致，可在老年人群结直肠肿瘤的筛查中发挥重要的作用。Objective To investigate the value of detecting methylated SEPT9 gene （mSEPTg） in serum and stool in screening colorectal cancer in elderly people. Methods Subjects were divided into three groups, the colorectal cancer group （n= 82）, the colorectal polyps group （n= 80） and the healthy control group （n= 100）. Real-time quantitative polymerase chain reaction （PCR） was used to detect mSEPT9 in peripheral blood and feces. The relationship between mSEPT9 and clinical and pathological characteristics of eolorectal cancer was analyzed. The agreement between serum and stool results was analyzed. Results The positive rate of serum mSEPT9 was 73.2~ in the colorectal cancer group, 6.3% in the colorectal polyps group and 4.0K in the healthy control group, with statistical significance between the colorectal cancer group and the other two groups （P〈0.01）. The sensitivity and specificity for plasma mSEPT9 detection in screening colorectal cancer were 73.2% and 95.6%, respectively. The positive rate was higher in patients with tumor maximum diameter over 3.0 em, invasion of the entire serosa layer, lower differentiation or advanced clinical staging （P〈0.05）. The detection results of plasma mSEPT9 were in complete agreement with those of stool mSEPT9 from the same patients. Conclusions The expression of mSEPT9 is high in elderly patients with colorectal cancer and has a high sensitivity and specificity for PCR detection. Complete agreement can be seen in results from plasma and feces. Detecting mSEPT9 can be a valuable approach to screening colorectal cancer in the elderly.

关 键 词：胞裂蛋白(SEPI)9 结直肠癌 甲基化 

分 类 号：R735.34[医药卫生—肿瘤]