壮肝逐瘀煎对肝纤维化大鼠血清Fas/FasL表达的影响  

Effect of Zhuanggan Zhuyu Decoction on serum Fas/FasL expression in rats with liver fibrosis

作　　者：林寿宁[1] 陈思羽[1] 程臣[2] 朱永苹[1] 

机构地区：[1]广西中医药大学附属瑞康医院,广西南宁530011 [2]广西中医药大学第一附属医院,广西南宁530023

出　　处：《现代中西医结合杂志》2015年第36期3994-3996,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(81260533)

摘　　要：目的观察壮肝逐瘀煎对肝纤维化大鼠血清Fas/Fas L表达的影响,探讨该组方抗肝纤维化的作用机制。方法将SD大鼠随机分为空白组、模型组和治疗组,除空白组外其余大鼠均采用CCl4复合因素法制作肝纤维化大鼠模型,模型制作成功后,治疗组给予壮肝逐瘀煎灌胃,空白组及模型组仅予等容量生理盐水灌胃。10周末所有大鼠经股静脉采血后处死并获取肝组织,HE染色光镜观察肝组织结构变化,ELISA法测定血清Fas、Fas L水平。结果光镜观察空白组肝小叶完整、结构正常,造模组肝纤维化程度明显,治疗组肝纤维化程度较轻;ELISA测定显示治疗组大鼠血清Fas和Fas L水平显著低于模型组(P均<0.05)。结论壮肝逐瘀煎可有效治疗大鼠肝纤维化,其作用可能是通过下调Fas、Fas L的表达,抑制肝星状细胞凋亡而实现的。Objective It is to observe the influence of Zhuanggan Zhuyu Decoction on serum Fas / Fas L expression in rats with liver fibrosis,and explore the action mechanism of anti-liver fibrosis. Methods The SD rats were divided into blank group,model group and treatment group,the rats model of liver fibrosis except blank group were established with CCl4 composite factor method. After the models were successfully,the rats were treated with Zhuanggan Zhuyu Decoction by gavage in treatment group,and with same amount of normal saline in the blank group and model group. At the end of the 10 th week the blood were collected through the femoral vein in all rats,then the animals were sacrificed to obtain liver tissue,the structural changes in liver tissue were observed by HE staining and light microscope,and the Fas,Fas L expression of the blood were detected by ELISA. Results Light microscopy showed that blank group with normal hepatic lobe and complete structure,modle group with significantly liver fibrosis,and treatment group with mild liver fibrosis; ELISA determination results showed that the serum Fas,Fas L expression in treatment gorup were singificantly lower than that in model group（ all P 〈 0. 01）. Conclusion Zhuanggan Zhuyu Decoction is effective in the treatment of liver fibrosis in rats,and its mechanism may be through reduce the expression of Fas,Fas L,and inhibit hepatic stellate cells apoptosis.

关 键 词：壮肝逐瘀煎 肝纤维化 FAS FAS L 大鼠 

分 类 号：R-332[医药卫生]