外源硫化氢对干旱条件下玉米幼苗抗氧化特性的影响  

Effects of exogenous hydrogen sulfide on the antioxidant characteristics of maize seedling under drought

作　　者：单长卷[1,2] 周岩[1,2] 赵元增[1,2] 

机构地区：[1]河南科技学院,河南新乡453003 [2]现代生物育种河南省协同创新中心,河南新乡453003

出　　处：《干旱地区农业研究》2015年第6期161-166,共6页Agricultural Research in the Arid Areas

基　　金：河南省教育厅科学技术研究重点项目(13A180302)

摘　　要：采用10%PEG模拟干旱胁迫的方法,研究了外源硫化氢对干旱条件下玉米幼苗根系和叶片抗氧化酶活性(SOD、POD、CAT和APX)、膜脂过氧化指标(MDA和膜透性)及叶片非酶抗氧化物质类胡萝卜素含量等抗氧化指标的影响。结果表明:与单独干旱处理相比,0.01、0.1、0.3、0.6 mmol·L-1和1.0 mmol·L-1Na HS使干旱条件下叶片类胡萝卜素含量分别增加了33.3%、63.3%、96.6%、80.0%和26.6%,使叶片POD活性分别增加了14.5%、36.6%、50.3%、91.1%和80.1%。此外,0.01、0.1 mmol·L-1和0.3 mmol·L-1Na HS均能显著提高叶片SOD、CAT、APX活性及根POD、APX活性,并显著降低根叶细胞质膜透性和MDA含量。0.6 mmol·L-1Na HS则使叶片SOD和CAT活性及根POD活性分别提高了7.4%、16.3%和45.0%,并使叶MDA含量及根细胞质膜透性分别降低了12.0%和18.0%。1.0 mmol·L-1Na HS却使根叶APX活性分别降低了13.3%和34.9%,并使叶细胞质膜透性及根MDA含量分别提高了130.1%和10.5%。以上结果说明,低浓度外源硫化氢可以有效提高干旱条件下玉米幼苗的抗氧化能力,尤其是0.3 mmol·L-1Na HS处理效果最好。In this paper,10% PEG was used to simulate drought stress,thereby studying the effects of exogenous hydrogen sulfide on the activities of antioxidant enzymes（ SOD,POD,CAT and APX）,the indexes related with membrane lipid peroxidation（ MDA and the permeability of cell membrane） in the roots and leaves and the carotenoid content in leaves of maize seedlings under drought stress. The results showed that,compared with drought stress alone,0.01、0.1、0.3、0.6 mmol·L-1and 1.0 mmol·L-1Na HS increased carotenoid content in leaf by 33.3%,63.3%,96.6%,80.0%,and 26.6%,respectively. Compared with drought stress alone,0.01,0.1,0.3,0.6mmol·L-1and 1.0 mmol·L-1Na HS increased the POD activity in leaf by 14.5%,36.6%,50.3%,91.1% and80.1%. Besides,pretreatment with 0.01、0.1 mmol·L-1and 0.3 mmol·L-1Na HS can significantly improve the activities of SOD and CAT in leaves,the POD activity in root and APX activity in root and leaf. Meanwhile,pretreatment with 0.01,0.1 mmol·L-1and 0.3 mmol·L-1Na HS reduced the permeability of cell membrane and the MDA content in root and leaf. 0.6 mmol·L-1Na HS could improve the activities of SOD and CAT in leaves and the activity of POD in root by 7.4%、16.3% and 45.0%,respectively. Meanwhile,0.6 mmol·L-1Na HS reduced the permeability of cell membrane of root and the MDA content in leaf by 12. 0% and 18.0%,respectively. However,1. 0mmol·L-1Na HS reduced the activity of APX in root and leaf by 13.3% and 34.9%,respectively. Due to the decrease in the APX activity,1.0 mmol·L-1Na HS increased the permeability of cell membrane of leaf and the MDA content in root by 130.1% and 10.5%,respectively. Collectively,these results suggest that pretreatment with lowconcentrations of hydrogen sulfide can effectively improve the antioxidant capacities of maize seedlings under drought stress,especially for 0.3 mmol·L-1Na HS.

关 键 词：硫化氢 玉米幼苗 干旱胁迫 抗氧化特性 

分 类 号：S513.01[农业科学—作物学] Q945.78[生物学—植物学]