芝麻茎点枯病菌两种细胞壁降解酶活性分析  

Analysis of the enzyme activity of two kinds of cell wall-degrading enzyme of Macrophomina phaseolinain sesame

作　　者：高树广[1,2] 赵辉[1] 李伟峰 王瑞霞 倪云霞[1] 杨修身[1] 刘红彦[1] 杨光宇 

机构地区：[1]河南省农业科学院植物保护研究所,郑州450002 [2]河南省周口市农业科学院,周口466001

出　　处：《植物保护》2015年第5期75-78,84,共5页Plant Protection

基　　金：现代农业产业技术体系建设专项资金(CARS-15-1-05)

摘　　要：为明确芝麻茎点枯病菌漆酶(Lac)和聚甲基半乳糖醛酸酶(PMG)活性,采用分光光度法测定了9株芝麻茎点枯病菌漆酶活性和聚甲基半乳糖醛酸酶活性。结果表明,芝麻茎点枯病菌胞内、胞外均能检测到漆酶,不同菌株之间漆酶活性差异显著;芝麻茎点枯病菌体外培养能持续产生聚甲基半乳糖醛酸酶,并且不同菌株之间聚甲基半乳糖醛酸酶活性差异显著。The purpose of this study is to examine and measure the enzyme activity.The Lac activity and PMG ac-tivity of nine strains of Macrophomina phaseolina were tested by spectrophotom.The results demonstrated that in-tracellular Lac activity and extracellular Lac activity could be detected in M.phaseolina ,and the Lac activity difference was significant among strains.PMG could be produced by M.phaseolina continuously during ten days, and the PMG activity difference was also significant among strains.

关 键 词：芝麻 茎点枯病菌 漆酶 聚甲基半乳糖醛酸酶 

分 类 号：S435.653[农业科学—农业昆虫与害虫防治]