阻断钙激活钾通道对宫颈癌细胞增殖及钾通道电流的影响  

Effects of blocking intermediate-conductance-Ca^(2+)-activated K^+ channels on proliferation of cervical cancer cells and potassium channel current

作　　者：刘玲[1] 聂丹[1] 范凌晔[1] 詹平[1] 钱燕萍[1] 毛熙光[1] 

机构地区：[1]泸州医学院附属医院,四川泸州646000

出　　处：《山东医药》2015年第43期1-4,共4页Shandong Medical Journal

基　　金：四川省科技厅应用基础研究基金资助项目(2008JY0014-1);四川省卫生厅科研基金资助项目(110373);四川省泸州市科技局科研项目(2013LZLY-J30)

摘　　要：目的观察钙激活性中电导钾离子通道(IKCa1)被阻断后对宫颈癌He La细胞增殖及IKCa1膜电位的影响。方法分别用IKCa1阻断剂克霉唑和RNA干扰法阻断He La细胞的IKCa1后,用RT-PCR技术检测He La细胞的IKCal mRNA,MTT法检测细胞OD值,膜片钳技术检测细胞IKCa1电流。结果克霉唑阻断IKCa1后,He La细胞的IKCa1 mRNA表达降低、IKCa1电流减弱、细胞OD值下降,与对照组相比,P均<0.05。RNA干扰法阻断IKCa1后,Hela细胞的IKCa1 mRNA表达下降、IKCa1电流减弱、细胞OD值降低,与空白对照组和阴性转染组相比,P均<0.05。结论阻断IKCa1能有效抑制He La细胞增殖,下调细胞IKCa1 mRNA的表达,降低其IKCa1电流;IKCa1通过调控He La细胞膜电位而影响其信号传递,调控He La细胞的增殖。Objective To investigate the effects of blocking intermediate-conductance-Ca^2＋-activated K^＋channels（ IKCa1） on the cell proliferation and the membrane potential changes of IKCa1 in cervical cancer He La cells. Methods IKCal of He La cells was blocked with IKCa1 channel inhibitor（ clotrimazole,CLT） and RNA interference,respectively.RT-PCR was used to detect the expression of IKCal mRNA in He La cells. The OD value of cells was detected by MTT,and IKCal current was detected by the patch clamp technique in He La cells. Results When IKCal was blocked with CLT in He La cells,the expression of IKCal mRNA was decreased,IKCal current wakened and the OD value of cells decreased as compared with that of the control group（ all P〈0. 05）. When IKCal was blocked by small interfering RNA technology in He La cells,the expression of IKCal mRNA reduced,IKCal current wakened,and the OD value of cells decreased as compared with that of the blank group and negative transfection group（ all P〈0. 05）. Conclusions Blocking IKCa1 could inhibit cell proliferation,down-regualte the expression of IKCa1 mRNA and cut down the IKCal current in He La cells. IKCa1 could regulate the cell proliferation of He La cells by directly regulating the membrane potential and thus affecting its signal transmission.

关 键 词：离子通道 钾离子通道 钙激活性中电导钾离子通道 钾离子通道阻断剂 克霉唑 小分子干扰RNA技术 宫颈癌细胞 He La细胞株 

分 类 号：R737.33[医药卫生—肿瘤]