左旋多巴治疗前后帕金森大鼠脑内γ-氨基丁酸A受体的变化  

The change of gamma aminobutyric acid A receptor on Parkinsons rat before and after L- dopa treatment

作　　者：王晓巍[1] 崔宏福[1] 姚素艳[2] 刘建生[1] 郑德宇[1] 

机构地区：[1]辽宁医学院解剖学教研室,辽宁锦州121000 [2]辽宁医学院病理生理学教研室,辽宁锦州121000

出　　处：《中国临床解剖学杂志》2015年第6期677-680,共4页Chinese Journal of Clinical Anatomy

基　　金：辽宁省自然科学基金(2013022066;2013022048);辽宁省教育厅一般项目(L2013335)

摘　　要：目的探讨帕金森病治疗前后脑内皮质、黑质、纹状体部位γ-氨基丁酸A受体(gamma aminobutyric acid A receptor,GABAAR)的分布变化规律。方法首先制备PD大鼠模型,经检测成模后,随机分为对照组和治疗组,分别给予生理盐水和左旋多巴甲酯+苄丝肼治疗4周,治疗后经行为学检测,选择成功的治疗后的PD大鼠,于治疗后4、8、12周分别留取大鼠脑皮质、黑质和纹状体部位的标本,进行免疫组化检测,对比PD大鼠治疗前后脑内上述部位GABAAR的分布、表达情况变化。结果治疗后4、8、12周的免疫组化检测结果经统计学分析,脑内黑质和皮质部位的GABAAR阳性表达的细胞治疗后比治疗前减少。而纹状体部位的GABA_AR阳性表达的细胞数量无明显变化。结论 (1)左旋多巴甲酯+苄丝肼治疗PD大鼠,可以使症状改善。(2)PD大鼠治疗前后脑内不同部位GABAAR的变化不同。Objective To investigate varying pattern of distribution of gamma aminobutyric acid A receptor （GABAAR） before and after treatment in the brain cortex, striatum, substantianigra on Parkinson＇s disease. Methodes First, the rat model of PD was made, after testing, were randomly divided into treatment group and control group. They were given normal saline and L-dopa methyl ester＋Benserazide for 4 weeks. After treatment the behavioral tests were performed. Cerebral cortex, striatum, substantianigra were harvested from rats after treatment of 4, 8, 12 weeks respectively. By immunohistochemistry, the changes in distribution and expression of content of GABAAR in the brain were compared before and after treatment. Results After treatment of 4, 8, 12 weeks immunohistochemical results was subjected through statistical analysis, which showed the content of the brain substantianigra and cortex GABAAR was decreased than before treatment, While the number of striatum showed no changes of GABAAR. Conclusion （1） With treatment of L-dopa methyl ester ＋ Benserazide, the PD symptoms can be improve. （2） The changes of GABAAR are different on different parts of PD rats before and after treatment.

关 键 词：帕金森病 Γ-氨基丁酸 Γ-氨基丁酸A受体 

分 类 号：R742.5[医药卫生—神经病学与精神病学]