ANA联合dsDNA在自身免疫疾病中的相关性和敏感性分析  

Analysis on the Correlation and Sensitivity of ANA combined with dsDNA in Autoimmune Diseases

作　　者：陈英杰[1] 曹令仪 雷泽洪[1] 冯武超[1] 钟玉琼[1] 

机构地区：[1]广东省江门市中心医院,广东江门529000

出　　处：《临床医学工程》2015年第12期1686-1687,共2页Clinical Medicine & Engineering

摘　　要：目的探讨ANA联合dsDNA在自身免疫疾病中的相关性和敏感性。方法选择2014年6月至2014年12月我院收治的自身免疫性疾病患者466例。采用间接免疫荧光法检测ANA、ELISA法检测dsDNA。观察ANA阳性患者ANA核型,SLE、MCTD等疾病患者ANA、dsDNA阳性率,以及不同疾病程度SLE患者血清ANA、dsDNA计分值。结果 184例ANA核型阳性患者中以核均质型构成比例最高,占33.15%,其次为胞浆型、核细颗粒型和核粗颗粒型,分别占20.11%、19.02%和16.30%。ANA阳性率从高到低分别是SLE、MCTD、PSS;dsDNA阳性率从高到低分别是SLE、MCTD。非活动期SLE患者ANA、dsDNA计分值明显低于中度活期组和重度活动期组,中度活动期组患者dsDNA计分值明显低于重度活动期组,差异具有统计学意义(P<0.05)。结论 ANA是诊断自身免疫疾病高度敏感的抗体,联合dsDNA可提高鉴别诊断准确性,为临床提供有价值的参考。Objective To study the correlation and sensitivity of ANA combined with dsDNA in autoimmune diseases. Methods 466 patients with autoimmune diseases treated in our hospital from June 2014 to December 2014 were selected. ANA was detected by indirect immunofluorescence, dsDNA was detected by ELISA method. The karyotype of ANA positive patients, the positive rate of ANA and dsDNA of SLE patients and MCTD patients, the scoring value of ANA and dsDNA of patients with different degree of SLE were observed. Results The proportion of nuclear homogeneous type in 184 ANA positive patients was the highest, accounting for 33,15%, followed by the cytoplasm type （20.11%）, nuclear fine particles type （19.02%） and coarse particles type （16.30%）. The positive rate of ANA from high to low was SLE, MCTD, PSS; the positive rate ofdsDNA from high to low was SLE, MCTD. The scoring value of ANA and dsDNA of SLE patients in inactive stage was lower than that of patients in moderate and severe stage, the scoring value of dsDNA of patients in moderate stage was lower than that of patients in severe stage （P 〈0.05）. Conclusions ANA is highly sensitive to the diagnosis of autoimmune diseases. ANA combined with dsDNA can improve the accuracy of differential diagnosis, and provide a valuable reference for clinical diagnosis.

关 键 词：ANA DSDNA 自身免疫疾病 相关性 敏感性 

分 类 号：R593.2[医药卫生—内科学]