厌氧氨氧化细菌和反硝化厌氧甲烷氧化细菌在岸边带土壤中的分布规律  

Distribution of anaerobic ammonia oxidizing bacteria and nitrite-dependent anaerobic methane oxidizing bacteria in soil profile in the riparian zone

作　　者：夏超[1] 祝贵兵[1] 邹雨璇 周蓉[1] 赵思研[1] 

机构地区：[1]中国科学院生态环境研究中心饮用水科学与技术重点实验室,北京100085

出　　处：《环境科学学报》2015年第12期3965-3975,共11页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.41322012;21277156);中国科学院战略性先导科技专项(No.XDB15020303);中国科学院青藏高原环境变化与地表过程重点实验室开放基金(No.TEL201304);环境模拟与污染控制国家重点联合实验室专项经费项目(No.14L01ESPC);北京市科技新星计划(No.2011095)~~

摘　　要：厌氧氨氧化（anaerobic ammonium oxidation,anammox）和反硝化厌氧甲烷氧化（nitrite-dependent anaerobic methane oxidation,n-damo）的发现打破了人们长久以来对生物氮、碳循环的传统认识.厌氧氨氧化细菌（anammox bacteria）和反硝化厌氧甲烷氧化细菌（n-damo bacteria）在水生态系统均有分布,并且在全球氮、碳循环中发挥重要作用.但它们在岸边带土壤中的存在和分布还不甚清楚.因此,本文对湖泊岸边带土壤中厌氧氨氧化细菌和反硝化厌氧甲烷氧化细菌的存在和分布进行了研究.基于厌氧氨氧化细菌hzs B基因（联氨合成酶关键基因）和M.oxyfera-like细菌16S rRNA基因的序列分析,分别证明了厌氧氨氧化细菌和M.oxyfera-like细菌在白洋淀湖泊岸边带深层土壤中的共同存在.厌氧氨氧化细菌hzs B基因定量PCR结果显示,其主要分布在地下水位附近及以下部分（40~100 cm）,而在表层（0~40 cm）土壤中未被检测到.M.oxyfera-like细菌16S rRNA基因定量PCR结果显示,不同深度的土壤均有M.oxyfera-like细菌分布,并且其丰度随着土壤深度的增加而递增.这些结果说明厌氧氨氧化细菌和M.oxyfera-like细菌在湖泊岸边带深层土壤中共同存在,并且有不同的分布规律.The discoveries of anaerobic ammonium oxidation （anammox） and nitrite-dependent anaerobic methane oxidation （n-damn） processes changed the understanding about biological nitrogen and carbon cycles. Both anammnx bacteria and n-damo bacteria have been detected in aquatic ecosystems, and they play important roles in nitrogen and carbon cycles. However, little is known about their occurrence and distribution in the riparian zone. In this study, the occurrence and distribution of anammox bacteria and n-alamo bacteria were investigated in a riparian zone of Baiyangdian Lake. The co-occurrence of anammox bacteria and n-damo bacteria in deep profiles of the riparian zone was confirmed by the amplification of functional hzsB gene （ hydrazine synthaseβsubunit） of anammox bacteria and M. oxyfera-likebacteria116S rRNA gene. Quantitative PCR analysis of hzsB gene demonstrated that anammox bacteria were not detected in the surface layers （0-40 cm） but presented in the deep soil （40- 100 cm） close to the groundwater level. The M. oxfferalike bacteria were presented in the whole soil profiles and the abundance increased in deep layers. The results suggested that anammox bacteria and M.oxyffea-like bacteria were concurrent in the deep soil layers （40- 100 cm） and showed different distribution patterns among soil profiles in the riparian

关 键 词：厌氧氨氧化(anammox) 反硝化厌氧甲烷氧化(n-damo) 共存 分布 岸边带 

分 类 号：X172[环境科学与工程—环境科学] X53