仔猪绿色气球菌的分离与鉴定  

Isolation and identification of Aeroeoccus viridans in piglets

作　　者：李浩欣[1] 段纲[1] 项勋[1] 杨志远[1] 朱旗[1] 邹丰才[1] 常华[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《中国兽医学报》2015年第12期1954-1958,共5页Chinese Journal of Veterinary Science

基　　金：云南省林业厅野生禽类重大动物疫病监测资助项目(K2400033);云南省高端人才引进计划资助项目(A3006886)

摘　　要：为了对某猪场下痢仔猪死亡的病料进行细菌分离培养,对其进行不同培养基培养及生化分析,PCR扩增细菌16S rRNA基因及测序分析,得到致病菌绿色气球菌。并对该菌进行药敏实验分析及绿色气球菌生长曲线的测定,结果显示这些菌株对头孢拉定、阿莫西林、强力霉素等药物敏感,而对呋喃唑酮等药物不敏感。本研究为我国绿色气球菌病细菌学检测以及此病的治疗提供了科学依据。To investigate the prevalence and the antibiotic resistance of a strain of Aeroeoccus viri- dans isolated from dead piglets,which were identified by biochemical assays, amplification of 16S rRNA genes by PCR and sequence analysis,and were evaluated for resistance to 20 antibiotics. The results showed that the isolate was susceptible to cefradine,amoxicillin and doxycycline, however, resistence to furazolidone. Results of this study provided a basis of check and treatment for the Aeroeoccus viridans of piglets in China.

关 键 词：仔猪 绿色气球菌 鉴定 16S RRNA 

分 类 号：S852.61[农业科学—基础兽医学] S855.99[农业科学—兽医学]