MTA2在食管鳞癌组织中高表达并与食管癌KYSE510细胞的增殖和迁移有关  

Up-regulation of MTA2 in esophageal squamous carcinoma tissues and its association with the proliferation and migration of esophageal carcinoma KYSE510 cells

作　　者：戴素丽 赵连梅[1] 崔雯萱 李磊[1] 彭克楠 史娟[2] 单保恩[1] 

机构地区：[1]河北医科大学第四医院科研中心,河北石家庄050011 [2]中国医学科学院基础医学研究所医学分子生物学国家重点实验室,北京100005

出　　处：《肿瘤》2015年第12期1335-1344,共10页Tumor

摘　　要：目的:探讨转移相关基因2(metastasis-associated gene 2,MTA2)在食管癌组织中的表达以及对食管癌KYSE510细胞增殖和迁移的影响。方法:选取2014年在河北医科大学第四医院进行手术切除的50例食管癌组织及其相应的癌旁组织,应用实时荧光定量PCR检测MTA2和转录因子特异性蛋白1(specifi city protein 1,Sp1)m RNA的表达。将MTA2-si RNA和阴性对照si RNA片段转染至食管癌KYSE510细胞后,应用MTS实验和Transwell实验检测KYSE510细胞的增殖、迁移和侵袭情况,应用蛋白质印迹法检测KYSE510细胞中与上皮-间质转化(epithelialmesenchymal transition,EMT)相关蛋白的表达情况。结果:食管癌组织中MTA2 m RNA的表达水平显著高于相应的癌旁组织(P<0.05),并且与肿瘤的TNM分期(P<0.05)、淋巴结转移和血管浸润有关(P值均<0.01)。食管癌组织中Sp1 m RNA的表达水平显著高于相应的癌旁组织(P<0.05),且MTA2 m RNA的表达水平与Sp1 m RNA的表达水平呈正相关(r=0.407,P=0.028)。MTA2-si RNA转染组细胞的增殖、迁移和侵袭能力显著低于阴性对照组(KYSE510细胞转染阴性对照si RNA片段)和空白对照组(KYSE510细胞未进行任何转染)(P值均<0.05)。MTA2-si RNA转染组细胞中E-cadherin的表达水平上调(P<0.05),而N-cadherin、vimentin和CD24蛋白的表达水平下调(P<0.05、P<0.01和P<0.05)。结论:食管癌中MTA2 m RNA的表达水平上调,可能与转录因子Sp1的表达水平升高有关。MTA2-si RNA可抑制食管癌KYSE510细胞的增殖、迁移和侵袭,这一作用可能与EMT有关。Objective： To investigate the expression leve esophageal cancer tissues and the effect of esophageal cancer KYSE51 0 cells. of metastasis-associated gene 2 （MTA2） in MTA2 on the proliferation and migration ofMethods： Fifty specimens of esophageal cancer tissues and adjacent para-cancerous tissues were obtained from patients who underwent surgery in Fourth Hospital, Herbei Medical University in 2014. The expression levels of MTA2 and transcription factor specificity protein 1 （Spl） mRNAs were examined by real-time fluorescent quantitative PCR. After KYSE510 cells transfection with MTA2-siRNA and negative control siRNA, the abilities of proliferation, migration and invasion were detected by MTS and Transwell assay, and the expressions of epithelial-mesenchymal transition （EMT）-related proteins in KYSE510 cells were detected by Western blotting.Results： The expression level of MTA2 mRNA in esophageal cancer tissues was significantly higher than that in adjacent para-cancerous tissues （P 〈 0.05）, and it was corrected with TNM staging, lymph node metastasis and blood vessel invasion （all P 〈 0.01）. The expression level of Spl mRNA in esophageal cancer tissues was significantly higher than that in adjacent para-cancerous tissues （P 〈 0.05）, and the expression level of Spl mRNA was positively correlated with MTA2 mRNA （r = 0.407, P = 0.028）. The capacities of proliferation, migration and invasion of KYSE510 cells in MTA2-siRNA transfection group were lower than those in negative control group （KYSE510 cells transfected with negative control-siRNA） and in blank control group （KYSE510 cells without any transfection） （all P 〈 0.05）. The expression level of E-cadherin protein in KYSE510 cells after transfection with MTA2-siRNA was up-regulated （P 〈 0.05）, and the expression level of N-cadherin, vimentin and CD24 proteins were down- regulated （P 〈 0.05, P 〈 0.01 and P 〈 0.05）.Conclusion： The expression level of MTA2 mRNA in esophageal cancer tissues is

关 键 词：食管肿瘤 细胞增殖 肿瘤转移 转移相关基因2 转录因子特异性蛋白1 

分 类 号：R735.1[医药卫生—肿瘤]