榴菌素产生菌——链霉菌CPCC 200532中fogacin的分离鉴定  

Identifi cation of fogacin from Streptomyces sp. CPCC 200532, a granaticin producer

作　　者：江冰娅[1] 赵薇[1] 李书芬[1] 孙桂芝[1] 武临专[1] 刘红宇[1] 余利岩[1] 山广志[1] 左利民[1] 

机构地区：[1]卫计委抗生素生物工程重点实验室中国医学科学院/北京协和医学院医药生物技术研究所,北京100050

出　　处：《中国抗生素杂志》2015年第12期913-917,共5页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(No.81302676);国家微生物资源平台(No.NIMR-2014-3)

摘　　要：目的对Streptomyces sp.CPCC 200532产生的榴菌素类似物进行分离鉴定。方法 Streptomyces sp.CPCC 200532发酵培养物经乙酸乙酯萃取后,用硅胶板薄层色谱和高效液相色谱对萃取物进行分析;通过质谱和NMR等对榴菌素类似物进行结构鉴定。结果从Streptomyces sp.CPCC 200532中发现了一个榴菌素类似物,结构与fogacin一致。结论从榴菌素产生菌中首次分离得到化合物fogacin;与榴菌素A相比,fogacin显示微弱的抗枯草芽孢杆菌和白色念珠菌活性。Objective To isolate granaticin analog（s） from Streptomyces sp. CPCC 200532. Methods Silica gel TLC and reversed phase HPLC were used to analyze the EtOAc extract of agar culture of Streptomyces sp. CPCC 200532. LC-MS and NMR were employed to identify granaticin analog（s）. Results A granaticin analog was characterized from Streptomyces sp. CPCC 200532, and it shared the same structure as fogacin. Conclusion Fogacin was isolated for the first time from a granaticin producer, and it should be a shunt metabolite in granaticin biosynthesis. Fogacin displayed much lower inhibitory activities against Bacillus subtilis CMCC 63501 and Candida albicans ATCC 10231 than granaticin A.

关 键 词：STREPTOMYCES sp.CPCC 200532 榴菌素 Fogacin 

分 类 号：Q939.9[生物学—微生物学]