血浆DLC-1甲基化与肝细胞性肝癌患者临床特征和预后的关系  

Expression and clinical significance of DLC-1 DNA methylation in hepatocellular cancer

作　　者：刘艳[1] 盖领[1] 茅国新[1] 

机构地区：[1]南通大学附属医院肿瘤化疗科,江苏226001

出　　处：《交通医学》2015年第5期443-446,共4页Medical Journal of Communications

基　　金：江苏省卫生厅资助项目(H200957)

摘　　要：目的:研究肝细胞性肝癌患者血浆DLC-1甲基化状态,研究其与各临床相关因素的关系及对肝细胞性肝癌患者预后的意义。方法:采用甲基化特异性PCR法检测肝细胞性肝癌患者和健康人群血浆中提取DNA的DLC-1甲基化状态,结合临床特征(性别、年龄、肝硬化、AFP水平、Hbs Ag及临床分期),分析DLC-1甲基化状态与肝细胞性肝癌患者复发转移和预后的关系。结果:96例肝细胞性肝癌患者血浆中DLC-1甲基化阳性率为30.2%,对照健康组无甲基化,差异有统计学意义(P<0.05)。肝细胞性肝癌患者血浆DLC-1甲基化状况与性别、年龄、抗-HBV、肝硬化无相关性(P>0.05),与HBs Ag、肿瘤大小、淋巴结转移及TNM分期相关(P<0.05)。DLC-1甲基化阳性组较阴性组转移复发率高,存活率较甲基化阴性组低。结论:DLC-1基因甲基化状态与肝细胞性肝癌发生发展及预后密切相关,对肝细胞性肝癌复发转移预测具有重要的价值,有望成为评估肝细胞性肝癌预后的有效指标。Objectives：To analyze the relationship between the promoter region DLC-1 DNA methylation status and the characteristics in hepatocellular cancer,and explore the role of DLC-1 DNA methylation in the occurrence and metastasis of hepatocellular cancer. Methods：96 cases of hepatocellular cancer specimens were selected,the methylation specific-PCR（MSP） was used to detect the tumor suppressor gene DLC-1 methylation in the above-mentioned tissues. To explore their roles of them in the occurrence and development of hepatocellular cancer, and the relationship associated with the clinical relevant factors. And to further analyze of the relationship between the promoter region of DLC-1 methylation status and the characteristics of methylation of the genome-wide in hepatocellular cancer.Disscussion the relationship between the methylation status with clinicopathologic factors（sex, age, AFP, differentiation degree, clinical stage,） and progression-free survival（PFS） as well as overall survival（OS） was analyzed. Results：The methylation status of DLC-1 in plasma of hepatocellular cancer patients was 30.1%. There were no correlations among the DLC-1 methylation status in hepatocellular cancer plasma and the patient＇s sex, age, cirrhosis and anti-HBV（P〈0.05）, but it was correlated with degrees of differentiation TNM staging. Multivariate analysis indicated that besides the tumor size, the methylation status is also an independent risk factor influencing recurrence, also independently correlated with TTP and OS in hepatic patients.Conclusions：DLC-1 methylation shows great importance in the field of judging the prognosis. Tumor suppressor gene DLC-1 methylation is an early molecular index of hepatocellular cancer.

关 键 词：甲基化 DLC-1 肝细胞性肝癌 预后 

分 类 号：R735.7[医药卫生—肿瘤]