Evaluation of the Anticonvulsant Activity of the Leaf Methanol Extract of Crassula arborescens （Mill.） Willd. （Crassulaceae） in Mice  

作　　者：George Jimboyeka Amabeoku Oluchi Nneka Mbamalu Tasneem Davids Samukelisiwe Fakude Anda Gqwaka Fiona Mbai Reighman Pieterse Aneesa Shaik 

机构地区：[1]Discipline of Pharmacology, University of the Western Cape, Cape Town 7535, Western Cape, South Africa

出　　处：《Journal of Pharmacy and Pharmacology》2014年第7期393-403,共11页药剂与药理学（英文版）

摘　　要：Crassula arborescens （Mill.） Willd. subsp. Arborescens is widely used for the treatment of various ailments including diarrhoea, corns, epilepsy and as a purgative. However, no information exists in any literature to verify the acclaimed effectiveness of C. arborescens in the treatment of the various ailments. The study, therefore, intended to investigate the anticonvulsant activity of the leaf methanol extract of C. arborescens in mice. Acute toxicity study and phytochemical qualitative analysis of the plant extracts were also carried out. Chemically-induced convulsion methods were used to assess the anticonvulsant activity of C. arborescens. Standard methods were used for the acute toxicity study and phytochemical analysis of the chemical components of the plant extract. PTZ （pentylenetetrazole）, bicuculline, picrotoxin, NMDLA （N-methyl-DL-aspartic acid） or strychnine produced tonic convulsions in all the mice used. Leaf methanol extract of Crassula arborescens, muscimol, phenobarbitone or diazepam significantly antagonised PTZ, bicuculline or picrotoxin-induced convulsion. C. arborescens or LY233053 significantly antagonised NMDLA-induced tonic convulsion. C. arborescens or phenobarbitone significantly antagonised strychnine-elicited tonic convulsion. Phenytoin or DMSO （dimethylsulfoxide） did not significantly affect the tonic convulsion produced by PTZ, bicuculline, picrotoxin, NMDLA or strychnine. The LDso value obtained from intraperitoneal administration of C. arborescens was 781.6 mg/kg while that following oral administration of the plant extract was over 4,000 mg/kg. The phytochemical qualitative analysis done showed the presence of flavonoids, tannins, reducing sugar, saponins and triterpene steroids. The data obtained in the study show that the leaf methanol extract of Crassula arborescens has anticonvulsant activity which may be underpinned by GABAergic, glutaminergic and glycinergic mechanisms. The high LDso value obtained following the oral administration of the plant extract shows

关 键 词：Crassula arborescens CRASSULACEAE anticonvulsant activity GABAERGIC glutamatergic and glycinergic mechanisms mice. 

分 类 号：R9[医药卫生—药学]