“氨在肝性脑病发病中的作用”家兔实验麻醉方法的改良  

Study of Intravenous Anesthesia in Rabbit Experiment of Exploring the Role of Ammonia Intoxication in Pathogenesis of Hepatic Encephalopathy

作　　者：吴卫[1] 何慧华[2] 董祁 

机构地区：[1]广州医科大学病理生理学教研室,广东省广州市511436 [2]广州医科大学机能学教研室,广东省广州市511436 [3]广州医科大学生理学教研室,广东省广州市511436

出　　处：《医学理论与实践》2015年第22期3029-3030,3035,共3页The Journal of Medical Theory and Practice

摘　　要：我国各大医科院校病理生理学传统的"氨在肝性脑病发病中的作用"实验中,由于家兔的上腹部大型手术只是在局部麻醉下进行,术中动物挣扎强烈,故此实验一直受到不少师生的抵触,个别院校甚至取消了该实验。本文旨在探讨如何将静脉麻醉方法有效运用于该实验。适量注射短效巴比妥类药硫喷妥钠,辅以局部麻醉,既能保证动物在安静状态下上腹部手术的顺利完成,又不会明显影响兔苏醒后氨的中枢毒性作用的观察。此改良麻醉方法值得在各大医科院校推广。The classical medical undergraduate experiment of exploring the role of ammonia intoxication in the pathogenesis of hepatic encephalopathy has been debated for years and even cancelled because in this experiment the rabbit will struggle strongly during the upper abdominal surgery that is carried out only under the local infiltration anesthesia.In this paper,intravenous anesthesia drugs（such as urethane,pentobarbitol sodium）are discussed,with the purpose to discover an appropriate ones to be applied in this experiment.It is suggested that intravenous administration of thiopental sodium（a short-acting barbiturate）,combined with local anesthesia,will not only make the rabbit to be more collaborative during the sugery but will also make the neuropsychical manifestations caused by acute ammonia intoxication duplicated after the awakening of rabbit be observed clearly.

关 键 词：短效巴比妥类 硫喷妥钠 急性氨中毒 家兔实验 肝性脑病 

分 类 号：R-332[医药卫生]