表皮生长因子对食管鳞癌细胞Eca109细胞周期及其调控因子的影响  

Effects of epidermal growth factor on cell cycle and cell cycle-related regulatory factors of human esophageal squamous cell carcinoma cell line Eca109

作　　者：李倩倩[1] 朱红[1] 王朝莉[2] 黎仕娟[1] 胡为民[1] 

机构地区：[1]川北医学院微生物学与免疫学教研室,南充637100 [2]川北医学院免疫学与分子生物学研究所,南充637007

出　　处：《中国免疫学杂志》2015年第12期1616-1620,共5页Chinese Journal of Immunology

基　　金：四川省科技厅重点科技自筹项目(No.2011JYZ023);川北医学院科研发展基金重点项目(No.CBY12-A-ZD19)资助

摘　　要：目的:探讨表皮生长因子(EGF)对食管鳞癌细胞Eca109细胞周期及相关调控因子的影响。方法:20 ng/ml重组人EGF(rh EGF)作用于血清饥饿的Eca109细胞24 h,采用流式细胞术检测EGF对Eca109细胞周期的影响,实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测p21CIP1/WAF1(p21)、p27KIP1(p27)mRNA的表达情况,Western blot检测p21、p27蛋白的表达情况。结果:EGF组和对照组G1期细胞所占比例分别为(54.90±0.82)%和(65.94±0.74)%(P<0.01);qRTPCR结果显示p21 mRNA表达水平EGF组明显高于对照组,p27 mRNA表达水平EGF组明显低于对照组(P<0.01);Western blot结果显示,p21蛋白表达水平EGF组明显高于对照组,p27蛋白表达水平EGF组明显低于对照组(P<0.01)。结论:EGF有利于Eca109细胞从G1期过渡到S期,促进细胞增殖,可能与调节p21、p27的mRNA和蛋白的表达相关。Objective： To investigate the effects of epidermal growth factor（ EGF） on cell cycle and cell cycle-related regulatory factors of human esophageal squamous cell carcinoma（ ESCC） cell line Eca109. Methods： Serum starved Eca109 cells were treated with 20 ng / ml recombinant human EGF（ rh EGF） for 24 h. The cell cycle phase distribution was detected by flow cytometry. The mRNA and protein expression levels of p21CIP1 / WAF1（ p21） and p27KIP1（ p27） were detected by real-time quantitative reverse transcription polymerase chain reaction（ qRT-PCR） and Western blot,respectively. Results： The proportions of G1 phase cells in EGF group and control group were（ 54. 90 ± 0. 82） % and（ 65. 94 ± 0. 74） %. The mRNA and protein expression levels of p21 in EGF group was significantly higher,and p27 was significantly lower than that in control group（ P 0. 01）. Conclusion： EGF facilitates G1-S phase transition,and promotes the proliferation of Eca109 cells,which may be associated with the up-regulation of p21 and down-regulation of p27.

关 键 词：表皮生长因子 食管鳞癌 ECA109细胞 细胞周期 P21CIP1/WAF1 P27KIP1 

分 类 号：R735.1[医药卫生—肿瘤]