阿维A酸联合IFNα-2a对皮肤鳞状细胞癌细胞SCL-12凋亡及caspases影响  

Effects of acitretin and IFNα-2a on apoptosis and caspases expressions in human cutaneous squamous cell carcinoma cell line SCL-12

作　　者：彭蕾蕾[1] 周国勇[2] 

机构地区：[1]江汉大学附属医院皮肤科,武汉430015 [2]江汉大学医学院药理教研室,武汉430056

出　　处：《中国免疫学杂志》2015年第12期1624-1627,共4页Chinese Journal of Immunology

摘　　要：目的:研究阿维A酸单用或联合IFNα-2a对皮肤鳞状细胞癌细胞SCL-12凋亡的诱导作用及其相关机制。方法:通过阿维A酸单用或联合IFNα-2a作用于体外培养的SCL-12细胞,同时采用ELISA法和吖啶橙染色法检测各组SCL-12细胞的凋亡情况及细胞凋亡的发生;Western blot观察caspase-3、caspase-8和caspase-9的表达水平。结果:与对照组相比,阿维A酸组、IFNα-2a组和联合用药组抑制率显著升高,且联合用药组作用强于阿维A酸组、IFNα-2a组;阿维A酸组、IFNα-2a组和联合用药组有显著的诱导细胞凋亡作用,联合用药组可观察到明显的细胞凋亡现象;阿维A酸组、IFNα-2a组和联合用药组中细胞的caspase-3、caspase-8和caspase-9均活化。结论:阿维A酸单用或联合IFNα-2a能抑制细胞SCL-12的增殖并能诱导其凋亡,且联合用药作用最强,其作用可能是通过外源性死亡受体途径和内源性线粒体途径共同作用产生。Objective： To study the effects of acitretin or IFNα-2a or both on the apoptosis and caspases expressions in humancutaneous squamous cell carcinoma cell line SCL-12. Methods： SCL-12 cells were cultured in vitro in the presence of or acitretin or IFNα-2a or both. Apoptosis was detected by enzyme-linked immunosorbent assay（ ELISA） in these cells. Apoptotic cells were observed by acridine orange staining. The protein expressions of caspase-3,caspase-8 and caspase-9 in SCL-12 cells were analyzed by Western blot. Results： The inhibition ratio of the cell growth significantly higher in groups of Acitretin or IFNα-2a or both than control group,and the effects of Acitretin combined with IFNα-2a stronger than the Acitretin or IFNα-2a. Caspase-3,caspase-8 and caspase-9 were significantly activated in combined group. The effects of Acitretin combined with IFNα-2a stronger than the Acitretin or IFNα-2a. Conclusion： Acitretin or IFNα-2a or both plays a central role in the apoptosis of cutaneous squamous cell carcinoma cells,which may be related to the Fas-way and mitochondria-way.

关 键 词：阿维A酸 IFNα-2a 鳞状细胞 细胞凋亡 

分 类 号：R363.1[医药卫生—病理学]