Facial Preparation of Magnetic Lipase as Efficient Biocatalyst to Resolute Esters Enantioselectively  

作　　者：ZHANG Yanmei QUAN Chunshan LIU Baoquan FAN Shengdi 

机构地区：[1]College of Life Science, Dalian Nationalities University, Dalian 116600, P R. China

出　　处：《Chemical Research in Chinese Universities》2015年第6期997-1002,共6页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China（No.21203017）, the Open Fund of State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences（No.N-11-3）, the Program for Liaoning Excellent Talents in University, China and the Fundamental Research Funds for the Central Universities, China（No.DC201502020304）.

摘　　要：Magnetic lipase was prepared by a facial and cost-effective method. Lipase from Pseudomonase cepacia was covalently linked to Fe304 nanoparticles, which were produced by co-precipitating Fe^2＋ and Fe^3＋ ions in ammonia solution and subsequent coating with 3,4-dihydroxylaldehyde. Magnetic Fe304 nanoparticles modified with 3,4-dihydroxylaldehyde bonded lipase efficiently（47 mg/g）. The resulting magnetic-lipase is active（0.68 μmol·min^-1·mg^-1） and enantioselective（e.e, of the product 〉98%） in the hydrolysis of racemic 1-phenylethyl acetate. Furthermore, as a heterogeneous catalyst, the magnetic-lipase can be magnetically recycled, and a retained activity of 48% was obtained even after 6 cycles.Magnetic lipase was prepared by a facial and cost-effective method. Lipase from Pseudomonase cepacia was covalently linked to Fe304 nanoparticles, which were produced by co-precipitating Fe^2＋ and Fe^3＋ ions in ammonia solution and subsequent coating with 3,4-dihydroxylaldehyde. Magnetic Fe304 nanoparticles modified with 3,4-dihydroxylaldehyde bonded lipase efficiently（47 mg/g）. The resulting magnetic-lipase is active（0.68 μmol·min^-1·mg^-1） and enantioselective（e.e, of the product 〉98%） in the hydrolysis of racemic 1-phenylethyl acetate. Furthermore, as a heterogeneous catalyst, the magnetic-lipase can be magnetically recycled, and a retained activity of 48% was obtained even after 6 cycles.

关 键 词：Covalent attachment 3 4-Dihydroxylaldehyde Enantioselective hydrolysis Lipases from Pseudomonasecepacia Fe3O4 nanoparticle RECYCLE 

分 类 号：O614.241[理学—无机化学] TS210.9[理学—化学]