二甲双胍对MCF-7肿瘤细胞系SP细胞的抑制作用  

Inhibitory effect of metformin on SP cells in tumor cell line MCF-7

作　　者：刘畅[1] 周恩相[1] 

机构地区：[1]福建省肿瘤医院,福州350014

出　　处：《现代医药卫生》2015年第24期3709-3711,共3页Journal of Modern Medicine & Health

摘　　要：目的研究二甲双胍、紫杉醇脂质体、他莫昔芬对人乳腺癌MCF-7肿瘤细胞系SP细胞的杀伤作用及相互的协同效应。方法将二甲双胍(0.3 mmol/L)、紫杉醇脂质体(60.0μg/L)、他莫昔芬(10.0μmol/L)分别对MCF-7肿瘤细胞进行单一或联合干预,并应用流式细胞仪分析SP细胞比例。结果空白对照组、二甲双胍组、紫杉醇脂质体组、他莫昔芬组、二甲双胍联合紫杉醇脂质体组、二甲双胍联合他莫昔芬组MCF-7肿瘤细胞系SP细胞比例各不相同,二甲双胍干预各组MCF-7肿瘤细胞系SP细胞比例分别较其余各组下降,差异均有统计学意义(P<0.01);二甲双胍干预各组MCF-7肿瘤细胞系SP细胞比例两两比较及非二甲双胍干预各组MCF-7肿瘤细胞系SP细胞比例两两比较,差异均无统计学意义(P>0.05)。结论 0.3 mmol/L二甲双胍可有效杀灭人乳腺癌MCF-7肿瘤细胞系SP细胞;二甲双胍与紫杉醇脂质体或他莫昔芬联用未表现出协同效应。Objective To investigate the killing effect of metformin, paclitaxel liposome and tamoxifen on SP cells in tu- mor cell line MCF-7 and their mutual synergistic effects. Methods The MCF-7 cells were performed the single or combined in- tervention with metformin （0.3 mmol/L）, paclitaxel liposome （60.0 μg/L） and tamoxifen （10.0 μmol/L） respectively. The propor- tion of SP cells was tested by flow cytometry. Results The proportions of SP cells in tumor cell line MCF-7 were different among the blank control group, mefformin group, paclitaxel liposome group, tamoxifen group, mefformin combined with paclitaxel lipo- some group and metformin combined with tamoxifen group ,which in the metformin intervention groups was decreased compared with other groups, the differences were statistically significant （P〈0.01） ;the proportion of SP cells had no statistical differences in the pairwise comparisons among the mefformin intervention groups and the non-metformin intervention groups （P〉0.05）. Conclu- sion 0.3 mmol/L metformin can effectively kill SP cells in tumor cell line MCF-7 ;the combined use of mefformin with paclitaxil liposome or tamoxifen has no synergistic effects.

关 键 词：乳腺 人 乳腺肿瘤 细胞系 肿瘤 二甲双胍 他莫昔芬 SP细胞 紫杉醇脂质体 

分 类 号：R730.21[医药卫生—肿瘤]