脂肪来源干细胞移植抑制脑缺血大鼠Neurocan的表达  

Adipose-derived stem cell transplantation for inhibiting Neurocan expression in rat with cerebral ischemia

作　　者：陈爱珍[1] 胡方进[1] 林菲菲[1] 

机构地区：[1]福建医科大学附属龙岩第一医院血液内科,福建龙岩364000

出　　处：《现代医药卫生》2015年第24期3714-3716,共3页Journal of Modern Medicine & Health

摘　　要：目的探讨脂肪来源干细胞(ADSC)移植对脑缺血大鼠神经蛋白聚糖(Neurocan)表达的影响。方法选取清洁级成年雄性SD大鼠54只,随机分为假手术组、模型组(大脑中动脉栓塞)和ADSC组(大脑中动脉栓塞后移植ADSC),每组18只。大脑中动脉栓塞模型采用改良Zea-Longa线栓制法,ADSC移植前用二脒基苯基吲哚标记,ADSC组于造模成功1 d后经侧脑室注入ADSC(1×106cells)。分别于术后7、14、28 d将大鼠断头取脑,采用免疫荧光、蛋白质印迹检测脑组织Neurocan蛋白表达情况。结果术后第7、14天模型组大鼠脑组织Neurocan表达明显升高,呈片状不规则分布,至28 d下降,但仍高于假手术组,差异均有统计学意义(P<0.05)。术后7、14 d ADSC组大鼠脑组织Neurocan表达明显减少,并持续至28 d,与模型组比较,差异均有统计学意义(P<0.05)。结论 ADSC移植后可减少脑缺血组织Neurocan表达,减少瘢痕产生,为后期神经轴突的成熟及功能建立提供了有利环境,可促进神经功能的恢复。Objective To investigate the effects of adipose-derived stem cell（ADSC） on the Neurocan expression in rat with cerebral ischemia. Methods 54 clean class adult male Sprague-Dawley rats were selected and randomly divided into the sham operation group,model group（middle cerebral arterial embolism） and ADSC group（ADSC transplantation after middle cerebral arterial embolism）,18 cases in each group. The middle cerebral arterial embolism model adopted the modified Zea-Longa line embolism method. Before ADSC transplantation,diamidino-2-phenylindole（DAPI） labeling was performed. The ADSC group was injected with ADSC（1×106cells） by lateral ventricle on 1 d after successful model establishment. Rats were sacrificed for taking the brain on postoperative 7,14,28 d and the Neurocan protein expression was detected by using the immunofluorescence and Western blot. Results The Neurocan expression of the brain tissues on postoperative 7,14 d in the model group was significantly increased and showed the sheet irregular distribution,which was decreased on 28 d,but still higher than that in the sham operation group,the differences were statistically significant（P0.05）. The Neurocan expression of the brain tissues on postoperative 7,14 d in the ADSC group was significantly decreased,moreover which continued to 28 d and showed the statistical difference compared with the model group（P0.05）. Conclusion The ADSC transplantation can decrease the Neurocan expression in the brain ischemic tissues and reduce the scar generation,which provides the enabling environment for late nerve axons maturity and function estab-lishment,and can promote the restoration of neural function.

关 键 词：干细胞 疾病模型 动物 颅内栓塞和血栓形成 蛋白聚糖类 神经轴突 

分 类 号：R743.31[医药卫生—神经病学与精神病学]