甘蓝型油菜花瓣发育过程中类胡萝卜素含量变化  

Carotenoids changes in petals during petal development of Brassica napus L.

作　　者：曾德志[1,2] 郭世星[1] 张星星[1] 杨华伟[2] 李霖超 牛应泽[1] 

机构地区：[1]四川农业大学油菜研究中心,四川温江611130 [2]自贡市农业科学研究所,四川自贡643000

出　　处：《广东农业科学》2015年第21期25-29,F0002,共6页Guangdong Agricultural Sciences

基　　金：四川省作物育种攻关项目(2011YZGG 005);四川省自贡市重点科技计划项目(2014NY19)

摘　　要：为了解不同颜色花瓣不同时期所含主要类胡萝卜素含量差异及其与花色的关系,将甘蓝型油菜花瓣发育划分为5个时期,采用分光光度法和HPLC法,对不同颜色油菜花瓣中总类胡萝卜素及其中3种主要胡萝卜素(叶黄素、番茄红素和β-胡萝卜素)含量进行测定分析。结果表明:油菜花瓣中总类胡萝卜素含量变化与其花瓣颜色变化一致,表明不同时期花瓣颜色变化与总类胡萝卜素含量变化密切相关;不同颜色花瓣中类胡萝卜素含量不同。黄色花中类胡萝卜素含量最高,白花油菜花瓣中类胡萝卜含量最低;叶黄素和β-胡萝卜素含量随花瓣发育逐渐减少,与总类胡萝卜素含量变化一致,而番茄红素含量变化为先增加后减少;不同颜色花瓣之间,3种类胡萝卜素中差异最大的是番茄红素,其在黄花中含量最高,在白花中含量最少。初步推测,总类胡萝卜素对油菜花瓣颜色的形成具有重要作用,而番茄红素则是花瓣颜色形成的主要影响因素。To observe the relationship of main carotenoid contents with different petal colors during the petal developmente in Brassica napus L..The petal development was divided into 5 stages in this research. At each stage the contents of total carotenoids,Xanthophyll,lycopene and β-carotene in petals were analyzed by spectrophotometry and HPLC. The results show that ：the change of total carotenoid content is consistent with the change of petal color during petal development,which shows that the changes of petal color during petal development is closely related to the total carotenoid content. And the carotenoid contents in different color petals were different. The carotenoid content in yellow petals was the highest,and the carotenoid content in white petals was the lowest. The contents of xanthophyll and β-carotene were decreasing during the petal development,which were consistent with the total carotenoid content. The content of lycopene experienced an obviously change of firstly increasing and then decreasing. Among different color petals,the content difference of lycopene was the largest,which was highest in yellow petals and lowest in white petals. So initially speculate that the total carotenoids play an important role in the formation of petal colors,and in which lycopene may be the major factor.

关 键 词：甘蓝型油菜 花瓣 花色 类胡萝卜素 

分 类 号：S184[农业科学—农业基础科学]