小白菊内酯对人前列腺癌细胞PC-3凋亡作用的机制探讨  

Study on the possible mechanism of parthenolide on apoptosis ofhuman prostate cancer cells PC-3

作　　者：艾俊 

机构地区：[1]曲靖市第一人民医院肿瘤外科,云南曲靖655000

出　　处：《四川生理科学杂志》2015年第4期171-174,共4页Sichuan Journal of Physiological Sciences

摘　　要：目的:探讨小白菊内酯诱导人前列腺癌细胞PC-3凋亡的作用及其机制。方法:体外培养人前列腺癌细胞PC-3,用不同浓度(0、10、20、40μmol·L-1)小白菊内酯作用PC-3细胞24、48、72h后,采用CCK8法观察PC-3细胞在增殖方面的变化,采用流式细胞术检测小白菊内酯对PC-3细胞凋亡的影响,采用RT-PCR技术检测PC-3细胞bax、bcl-2的mRNA表达水平。结果:经小白菊内酯处理后,CCK8检测显示PC-3细胞增殖率显著下降(P<0.05),流式细胞术检测显示PC-3细胞凋亡率显著升高(P<0.05),RT-PCR检测显示PC-3细胞bax mRNA表达水平增高(P<0.05)、bcl-2mRNA表达水平降低(P<0.05)。结论:小白菊内酯通过诱导凋亡能抑制人前列腺癌细胞PC-3的增殖,这可能与其上调bax和降低bcl-2mRNA表达有关。Objective：To explore the underlying mechanism responsible for parthenolide-induced apoptosis on human prostate cancer cell PC-3in vitro.Methods：Human prostate cancer cell PC-3were cultured in virto.Different concentrations of parthenolide（0,10,20 and 40μmol·L-1）were added to PC-3cell.The inhibitory effect of parthenolide on PC-3cell was determined by CCK8 assay,while apoptosis was analyzed by flow cytometry.Next,the mRNA levels of baxand bcl-2were tested by RT-PCR.Results：After parthenolide treatment,CCK8 test indicated parthenolide could inhibit the proliferation of PC-3cell（P〈0.05）.The increased apoptotic rates by parthenolide was found in PC-3cell by flow cytometry（P〈0.05）.The results from RT-PCR suggested that parthenolide treatment contributed to the reduced bcl-2mRNA level and the elevated bax mRNA expression in PC-3cell.Conclusions：Parthenolide could significantly inhibit the proliferation of human prostate cancer cell PC-3by induction of apoptosis,which may be associated with activating bax mRNA expression and inhibiting bcl-2mRNA expression.

关 键 词：小白菊内酯 PC-3细胞 凋亡 BCL-2MRNA BAX mRNA 

分 类 号：R737.25[医药卫生—肿瘤]