硫酸化茯苓多糖对MPP^+诱导的PC12细胞损伤的保护作用研究  

The Protective Effect of Sulfation Pachymaran on the Damage of PC12 Cells Induced by MPP^+

作　　者：高贵珍[1] 吴超[1] 陈哲[1] 刘小阳[1] 

机构地区：[1]宿州学院生物与食品工程学院,安徽宿州234000

出　　处：《淮北师范大学学报（自然科学版）》2015年第4期52-58,共7页Journal of Huaibei Normal University：Natural Sciences

基　　金：安徽省教育厅自然科学基金资助项目(KJ2013A242)

摘　　要：为探究硫酸化茯苓多糖(SP)对MPP+损伤的PC12细胞的保护作用,通过一定浓度的SP与PC12细胞共培养,检测SP的毒性作用;以MPP+损伤PC12细胞作为帕金森病(PD)细胞模型,MTT法检测细胞活力,显微镜观察细胞形态,紫外分光光度计法测定SOD、GSH-Px、LDH活性以及MDA含量.结果表明:SP浓度在0.5~4 mg/m L范围内对PC12细胞没有毒性;200μmol/L MPP+作用于PC12细胞24 h建立损伤模型;MPP+模型组细胞数量明显减少,细胞突起收回或消失,胞体变小、变圆;LDH释放量、MDA含量显著增加(均为P<0.01),SOD和GSH-Px活性极显著降低(均为P<0.01).与模型组相比,SP浓度在2~4 mg/m L时,细胞活力增大(P<0.05),细胞数量显著上升,胞体变大,贴壁能力恢复明显;LDH的释放量、MDA含量显著下降(均为P<0.01),SOD和GSH-Px活性显著提高(均为P<0.01).一定浓度的SP能够抑制MPP+对PC12细胞的氧化应激损伤.In order to explore the protective effect of sulfation pachymaran on the damage of PC12 cells induced by MPP＋,PC12cells were co-cultured with different concentrations of SP to determine its potential toxicity;Using MPP＋ induced PC12 cells as the cell model of Parkinsons disease,the cell viability was detected by MTT assay.Morphological changes were observed under microscopy,and the contents of SOD,GSH-Px, LDH,and MDA were tested by UV spectrophotometer.The result showed that no potential cell toxicities of SP in the range of 0.5-4 mg/mL were observed;PC12 cells treated with 200 umol/L MPP＋ for 24 h were used as damage and model;the number of PC12 cells in MPP＋ induced model group obviously decreased;cell processes shrunk or vanished,cell bodies became smaller,meanwhile the contents of LDH and MDA were significantly increased （all P〈0.01）,while the activities of SOD and GSH-Px were markedly decreased （all P〈0.01）. Compared with the model group,by treatment with 2-4 mg/mL SP,cell viability was significantly increased （P〈0.05）,the number and volume of cells were also increased,and the adherent abilities of cells were recovered obviously.The levels of LDH and MDA were significantly decreased （all P＆lt;0.01）,whereas the activities of SOD and GSH-Px were markedly increased （all P〈0.01）. SP at certain concentrations may inhibit MPP＋induced oxidative stress damage in PC12 cells.

关 键 词：硫酸化茯苓多糖 PC12细胞 MPP+ 抗氧化活性 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]