下调RbAp48表达可抑制人宫颈癌MS751细胞株的迁移侵袭能力  

Effect of RbAp48 knockdown on migration and invasion of human cervical cancer cell line MS751 in vitro

作　　者：钟晶晶[1] 杨旭锐[1] 麦梅清 王旦旦[1] 吕琳[1] 饶进军[1] 

机构地区：[1]南方医科大学药学院,广东广州510515

出　　处：《南方医科大学学报》2015年第11期1564-1569,共6页Journal of Southern Medical University

基　　金：国家自然科学基金(81473246);广东省教育部产学研结合项目(2012B091100465)~~

摘　　要：目的探讨下调Rb Ap48表达对人宫颈癌细胞迁移侵袭的影响及其作用机制。方法 si RNA下调Rb Ap48表达,以划痕实验、Transwell小室检测下调Rb Ap48表达后对人宫颈癌细胞株MS751迁移侵袭的影响,Western bolt检测Vimentin、N-cadherin、E-cadherin、Snail、Twist、MMP-2、TIMP-2蛋白的表达。结果下调Rb Ap48表达后MS751细胞的迁移和侵袭数均明显减少(P<0.01);其间质细胞表型蛋白Vimentin、N-cadherin、MMP-2表达明显受到抑制;上皮细胞表型蛋白E-cadherin、TIMP-2表达明显升高,显示MS751细胞的上皮-间充质样变(EMT)受到抑制。Snail、Twist表达水平也发生显著下调。结论下调Rb Ap48表达能有效抑制宫颈癌MS751细胞株的上皮-间充质样变,降低细胞的迁移侵袭能力;其作用机制与降低Snail、Twist的表达有关。Objective To investigate the effect of Rb Ap48 knockdown on the migration and invasion of human cervical cancer cells and explore the mechanism. Methods A small interference RNA（si RNA） was used to knock down the expression of Rb Ap48 in MS751 cells. The changes in cell migration and invasion were evaluated using wound healing assay and Transwell assay, respectively, and the expressions of Rb Ap48, vimentin, N-cadherin, E-cadherin, Snail, Twist, MMP-2 and TIMP-2 were determined with Western blotting. Results After si RNA-mediated Rb Ap48 knockdown, MS751 cells showed a significantly reduced expression of Rb Ap48 with significantly suppressed cell migration and invasion（P〈0.01）. Rb Ap48 knockdown induced obvious down-regulation of the expressions of interstitial cell phenotype proteins vimentin, N-cadherin, and MMP-2and up-regulation of epithelial cell phenotype proteins E-cadherin and TIMP-2, suggesting the inhibition of epithelialmesenchymal transition of the cells. The expressions of Snail and Twist were significantly down-regulated in the cells following Rb Ap48 knockdown. Conclusion Knockdown of Rb Ap48 can significantly inhibit epithelial-mesenchymal transition and suppress the migration and invasion of cervical cancer cell line MS751, the mechanism of which may involve the down-regulation of Snail and Twist expressions.

关 键 词：RbAp48 宫颈癌 迁移 侵袭 上皮-间充质样变 

分 类 号：R737.33[医药卫生—肿瘤]