hMSH2和nm23在肠癌中的表达及临床意义  

The expression of hMSH2 and nm23 in intestinal cancer and its clinical significance

作　　者：沈冬[1] 张瑜[2] 

机构地区：[1]江苏省苏州市中医医院检验科,215000 [2]江苏省苏州市立医院北区检验科,215008

出　　处：《检验医学与临床》2015年第24期3655-3656,3659,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨人MutS同源蛋白2(hMSH2)和转移抑制基因nm23在肠癌中的表达、相互关系及其临床意义。方法选取未经过术前放、化疗的肠癌标本48例及癌旁正常黏膜组织15例,XP免疫组织化学法检测hMSH2和nm23蛋白的表达,分析hMSH2和nm23蛋白的表达与肠癌临床病理特征的关系及其相互关系。结果肠癌组织hMSH2和nm23蛋白阳性表达率分别为35.4%、62.5%,均显著低于正常肠黏膜组织(93.3%),差异有统计学意义(P<0.05);nm23表达与患者性别、年龄及肿瘤分化程度无关,但与肿瘤浆膜浸润、肠癌淋巴结转移密切相关(P<0.05);hMSH2与临床病理参数无相关性;nm23与hMSH2蛋白的表达呈正相关(P<0.05)。结论nm23蛋白质水平与恶性程度相关,可作为预测入侵和转移的评判指标。hMSH2蛋白的表达与nm23蛋白合并后的表达对肠癌预后有一定指导意义。Objective To study the expression of hMSH2 and nm23in intestinal cancer,and to explore their inter-relationship and clinical significance.Methods XP immunohistochemistry was used to detect the expression levels of hMSH2 and nm23in samples including 48 cases of intestinal cancer tissues without radiotherapy or chemotherapy and 15 cases of adjacent normal colon tissues.The relevance of the expression levels of nm23 and hMSH2and their correlations to the clinicophathologic features of intestinal cancer were analyzed.Results The positive rates of hMSH2 and nm23were respectively 35.4% and 62.5% in intestinal cancer tissues,which were significantly lower than those in adjacent normal colon tissues（93.3%）,with statistical difference（P〈0.05）.The expression level of nm23 was not significantly associated with the genders,ages or differentiation of the patients,however,it was closely associated with serous coat infiltration and lymphatic metastasis of intestinal cancer（P〈0.05）.The expression level of hMSH2 was not significantly correlated with these clinicopathologic features.There was a positive correlation between the expression of nm23 and hMSH2in intestinal cancer（P〈0.05）.Conclusion The expression level of nm23 was related with the degree of malignancy,which could be used as a potential index to predict the invasion and metastasis.The expression of hMSH2 and nm23had certain guiding significance for the prognosis of intestinal cancer.

关 键 词：NM23 HMSH2 肠癌 肿瘤转移 

分 类 号：R737.9[医药卫生—肿瘤]