趋化素通过上调磷酸化JNK促进小鼠血管平滑肌细胞的增殖  

Chemerin promotes proliferation of mouse vascular smooth muscle cells by up-regulating p-JNK

作　　者：熊玮 董少红 张键[2] 李江华 廖碧红 庞新利 罗林杰 

机构地区：[1]暨南大学第二临床医学院/深圳市人民医院心内科,广东深圳518020 [2]中国科学院深圳先进技术研究院,广东深圳518055

出　　处：《中国病理生理杂志》2015年第12期2282-2286,共5页Chinese Journal of Pathophysiology

基　　金：深圳市重点资助科技计划(No.201201022)

摘　　要：目的:利用慢病毒构建趋化素基因缺陷性小鼠血管平滑肌细胞株,观察沉默趋化素基因后血管平滑肌细胞的增殖情况并探讨其机制。方法:将正常血管平滑肌细胞、趋化素基因干扰对照血管平滑肌细胞株和趋化素基因缺陷性血管平滑肌细胞株分成正常组、增殖组、对照组和沉默组,增殖组加入血小板源性生长因子BB促进增殖,采用细胞计数和Brd U法检测血管平滑肌细胞增殖,采用Western blot检测丝裂原激活蛋白激酶(MAPK)信号通路的蛋白水平。结果:增殖组血管平滑肌细胞的细胞数目和Brd U的A值显著高于正常组(P<0.05);与正常组相比,沉默组的血管平滑肌细胞数目和Brd U的A值显著降低(P<0.05);与此同时,对照组与正常组之间的血管平滑肌细胞增殖情况无明显差异。各组血管平滑肌细胞之间的p-ERK1/2、ERK1/2、p-p38 MAPK和p38 MAPK水平无明显变化,增殖组血管平滑肌细胞的p-JNK蛋白表达增加,而沉默组血管平滑肌细胞的p-JNK水平下降。结论:趋化素具有促进小鼠血管平滑肌细胞增殖的作用,该作用可能与p-JNK表达上调相关。AIM： To investigate the proliferation property of stable chemerin gene knockdown vascular smooth muscle cells（ VSMCs） and to explore its mechanism. METHODS： The normal VSMCs,chemerin gene interfering control VSMCs and stable chemerin gene knockdown VSMCs were divided into normal group,PDGF group,control group and knockdown group. The VSMCs in PDGF group were given platelet-derived growth factor-BB（ PDGF-BB） to initiate proliferation. The cell counting and Brd U assay were employed to investigate the proliferation property of VSMCs. The mitogenactivated protein kinase（ MAPK） signal pathway was determined by Western blot. RESULTS： The cell number and Brd U A value in PDGF-BB-treated VSMCs significantly increased as compared with the normal VSMCs（ P〈0. 05）. Compared with the normal VSMCs,the chemerin knockdown VSMCs showed obviously decreased cell number and Brd U A value（ P〈0. 05）. Simultaneously,no significant difference in the proliferation of VSMCs between the normal VSMCs and the control VSMCs was observed. No significant difference of the protein levels of p-ERK1 /2,ERK1 /2,p-p38 MAPK and p38 MAPK among 4 kinds of VSMCs was found. The protein level of p-JNK in PDGF-BB-treated VSMCs was up-regulated,while it was down-regulated in chemerin knockdown VSMCs compared with the normal VSMCs. CONCLUSION： Chemerin promotes the proliferation of mouse vascular smooth muscle cells by up-regulating p-JNK production.

关 键 词：血管平滑肌细胞 趋化素 细胞增殖 C-JUN氨基末端激酶 

分 类 号：R363[医药卫生—病理学]