宫颈癌中Snail、Slug和KAI1的表达及临床意义  

Expressions of Snail, Slug and KAI1 proteins in cervical carcinoma and their clinicopathological significance

作　　者：龚晓萌[1] 陶仪声[1] 周蕾[1] 俞岚[1] 武世伍[1] 宋文庆[1] 王丹娜[1] 承泽农[1] 

机构地区：[1]蚌埠医学院第一附属医院病理科//蚌埠医学院病理教研室,安徽蚌埠233004

出　　处：《南方医科大学学报》2015年第12期1733-1738,共6页Journal of Southern Medical University

基　　金：安徽省自然科学基金(1408085MH147)

摘　　要：目的检测宫颈癌中上皮间充质转化(EMT)相关标记物Snail、Slug和转移抑制因子KAI1的表达,并分析它们的相互关系及其临床病理意义。方法利用免疫组化Elivision TM plus法检测154例宫颈鳞状细胞癌(CSCC)、50例宫颈上皮内瘤变(CIN)和40例正常宫颈上皮组织中Snail、Slug和KAI1蛋白的表达。结果 Snail、Slug和KAI1在CSCC组、CIN组和对照组的阳性表达率分别为66.2%、66.9%、43.5%;32.0%、34.0%、64.0%和0%、2.5%、95.0%,两组相互之间差异有统计学意义(P<0.05)。Snail、Slug和KAI1蛋白的表达均与CSCC肿瘤组织的分化程度、淋巴结转移与否、浸润深度、FIGO分期及术后生存期均有关(P<0.05);Snail蛋白的表达与Slug蛋白的表达呈正相关关系(r=0.752,P<0.001),Snail和Slug蛋白的表达均与KAI1蛋白的表达呈负相关关系(P<0.001)。Kaplan-Meier生存分析表明,Snail和Slug蛋白表达阳性的患者其生存率明显低于其阴性表达的患者(P<0.001);KAI1蛋白表达阳性的患者其生存率明显高于其阴性表达的患者(P<0.001)。Cox回归分析显示Snail和KAI1蛋白的阳性表达及FIGO分期是影响CSCC患者术后生存的独立预后因素。结论 Snail、Slug和KAI1蛋白的表达水平在CSCC组织中与肿瘤组织的分化程度、FIGO、浸润深度、淋巴结转移与否及预后等均有关;在CSCC组织中早期联合检测Snail、Slug和KAI1对判断预后有价值。Objective To explore the expression of Snail and Slug in primary cervical squamous cell carcinoma（CSCC） and their relationship with KAI1 expression. Methods The expressions of Snail, Slug, and KAI1 proteins were examined by immunohistochemistry in 154 specimens of CSCC tissues, 50 specimens of cervical intraepithelial neoplasm（CIN）, and 40 specimens of normal cervical tissues. Results The positivity rates of Snail, Slug, and KAI1 expression were 0%, 2.5%, and 95.0%in normal cervical tissues, 32.0%, 34.0% and 64.0% in CIN tissues, and 66.2%, 66.9%, and 43.5% in CSCC tissues, respectively,showing significant differences in the rates among the 3 groups（P〈0.05）. The expressions of Snail, Slug, and KAI1 were significantly correlated with the histological grades of the tumor, depth of invasion, lymph node metastasis, International Federation of Gynecology and Obstetrics（FIGO） stages, and postoperative survival time（P〈0.05）. The expressions of Snail and Slug were positively correlated（r=0.752, P〈0.001）, and both of them were negatively correlated with the expression of KAI1（P〈0.001）. Kaplan- Meier analysis showed that patients positive for Snail and Slug had significantly lower survival rates than the negative patients（P〈0.001）, while a positive expression of KAI1 was associated with a higher survival rate of the patients. Cox regression analysis identified Snail, KAI1, and FIGO stage as independent factors that affected the outcomes of CSCC（P〈0.05）.Conclusion The expressions of Snail, Slug, and KAI1 are related to the tumor grade, FIGO stage, invasive depth, lymph node metastasis, and prognosis of CSCC, and their combined detection can help estimate the outcomes of the patients.

关 键 词：宫颈肿瘤 SNAIL SLUG KAI1 预后 

分 类 号：R737.33[医药卫生—肿瘤]