一株具有降解性能的巨大芽孢杆菌的鉴定与发酵条件优化  

Identification and Optimization of Fermentation Condition for Bacillus megaterium from Sea Cucumber Ponds

作　　者：辛美丽 吕芳 孙福新 

机构地区：[1]山东省海洋生物研究院,山东青岛266104 [2]青岛市大型海藻工程技术研究中心,山东青岛266104

出　　处：《广西科学院学报》2015年第4期247-252,共6页Journal of Guangxi Academy of Sciences

基　　金：海洋公益性行业科研专项经费项目(201305005);海洋经济创新发展区域示范项目;青岛市市南区科技发展资金项目(2013-12-005-SW)资助

摘　　要：【目的】对从刺参养殖池塘中分离得到的降解菌株B11进行菌种鉴定,并对其发酵条件进行优化。【方法】经生化及16SrDNA鉴定该菌种的种属;利用单因素筛选和正交试验设计对其发酵条件进行研究。【结果】经鉴定该菌株为一株巨大芽孢杆菌,最优发酵条件是装液量为70mL/250mL,转速为180r/min,培养温度为28℃,pH值为8。【结论】菌株B11在最优发酵条件下生长良好,满足工业化大规模扩大培养所需条件,有较好的应用前景。【Objective】A bacterium named B11 capable of organic-pollutants degradation,isolated from sea cucumber external culturing ponds,was identified and the liquid fermentation condition of B11 was optimized.【Methods】The genus of B11 was studied through physiological and bio-chemical tests and 16 SrDNA sequence similarity analysis.The condition of liquid fermentation for bacterium B11 were optimized by mono-factor experiment and uniform design.【Results】B11was preliminarily identified Bacillus megaterium.The optimized fermentation condition was at pH 8with 70 mL production medium inoculated with 5%seed culture and incubated at 28℃,180r/min.【Conclusion】B11grew well under optimal fermentation conditions and it could satisfy the requirement for industrial large-scale cultivation,which shows a good prospect for industrial application in future.

关 键 词：巨大芽孢杆菌 刺参 正交实验 发酵条件 

分 类 号：S917.1[农业科学—水产科学]