光动力学疗法的抑瘤作用和免疫效应的实验研究  

Experimental Studies on Photodynamic Therapy's Effect

作　　者：徐静[1] 孙新梅[2] 陶露[1] 

机构地区：[1]空军勤务学院门诊部 [2]95958部队门诊部

出　　处：《中国继续医学教育》2015年第32期204-207,共4页China Continuing Medical Education

摘　　要：目的探讨光动力学疗法(PDT)抑瘤效应的免疫机理,以及PDT联合肿瘤局部注射卡介苗(BCG)的治疗效果。方法建立小鼠Heps肝癌移植瘤模型96只,随机分为对照组、PDT组、BCG组和联合组。观测各组移植瘤体积的变化。采用酶联免疫吸附实验(ELISA),测定各组荷瘤小鼠血清中白细胞介素(interleukin,IL)-1β的变化。结果 (1)各治疗组治疗后7 d的移植瘤体积较对照组均明显缩小(P<0.01)。联合组治疗后7 d的肿瘤体积较单纯PDT组明显缩小(P<0.05)。(2)ELISA结果显示,PDT组和联合组治疗后2 h、24 h以及7 d荷瘤小鼠血清中IL-1β的含量,均高于对照组和BCG组(P<0.01)。联合组治疗后2 h、24 h以及7 d荷瘤小鼠血清中IL-1β的含量,超过单纯PDT组(P<0.05)。结论结果显示PDT对强化荷瘤宿主免疫力有显著作用。使肝癌移植瘤体积明显缩小。PDT联合局部注射BCG的治疗方法的疗效优于单用PDT和单用BCG。Objective To invest the local immune effect of photodynamic therapy（PDT） and the effect of PDT combined with local injection of BCG. Methods 96 tumor-bearing models were established with liver carcinomas subcutaneously transplanted in mice. The mice were divided randomly into four groups： control group, PDT group, BCG group, and PDT plus BCG group. Tumor size was measured in each group. IL-1β was measured with enzymelinked immunosorbent assay（ELISA） kit. Results（1） The tumor volume of PDT group, BCG group, and PDT plus BCG group was smaller than that of the control group on the 7 days after therapy（P〈0.01）. The tumor volume of PDT plus BCG group was smaller than that of the PDT group on the 7 days（P〈0.05）.（2） At 2 hours, 24 hours and 7 days after therapy, ELISA results showed that compared with control group and immune therapy only group, the abilities of PDT group and PDIT group to secrete cell factors IL-1β increased obviously. And there was statistic significance（P〈0.01）. At 24 hours and 7 days after therapy, ELISA results showed that compared with PDT only group, the abilities of PDIT group to secrete cell factors IL-1β increased obviously. And there was statistic significance（P〈0.05）. Conclusion PDT had the inhibitory effect on tumor growth of mice Heps cell line, and spontaneously could induce the systemic immune effects. The therapeutic effects of PDT combined with BCG were superior to PDT only and BCG only.

关 键 词：Heps肝癌细胞株 光动力学疗法 卡介苗 细胞因子 小鼠 

分 类 号：R730.5[医药卫生—肿瘤]