睡眠剥夺对低压低氧大鼠认知功能影响的实验研究  

Experimental Study of Effects of Sleep Deprivtion on Cognitve Impairment in Hypobaric Hypoxia Rats

作　　者：杨俊[1] 勇彤[1] 胡晓霞[1] 魏才[1] 梁丹[1] 努尔比亚[1] 

机构地区：[1]新疆兰州军区乌鲁木齐总医院干三科,830000

出　　处：《高原医学杂志》2015年第3期1-3,共3页Journal of High Altitude Medicine

基　　金：全军医药卫生科研基金兰州军区面上项目资助;课题名称:急性高原暴露人群睡眠障碍对认知功能的影响及机制研究;编号:clz12ja15

摘　　要：目的:观察睡眠剥夺对低压低氧环境急性暴露大鼠认知功能的影响。方法:采用低压低氧舱模拟海拔4 000m低压低氧环境,对S-D大鼠分别进行24h、48h、72h睡眠剥夺,通过水迷宫实验观察认知功能的变化,用免疫荧光法观察海马区Bax、Caspase-3的表达。结果:1与对照组比较,睡眠剥夺24h、48h、72h,均延长了水迷宫实验S-D大鼠的平均逃逸潜伏期(P<0.01),暴露的时间越长,潜伏期越长(P<0.05);2与对照组比较,睡眠剥夺72h组海马CA1/CA3区caspase-3阳性的神经元明显增多(P<0.05),睡眠剥夺48h组海马CA1/CA3区Bax阳性的细胞数明显增多(P<0.01)。结论:睡眠剥夺可加重急性低压低氧暴露大鼠认知功能的损害,其机制可能与海马区细胞的凋亡分子表达有关。Objective： To observe the effects of sleep deprivation on cognitive impairment in rats exposed to acute hypobaric hypoxia environment. Methods： The low pressure and low oxygen cabin were used to simulate 4000 m altitude hypobaric hypoxia environment. S- D rats were treated with 24 h,48h,72 h sleep deprivation. The changes of cognitive function were observed by water maze test,the expression of Bax,Caspase- 3 in hippocampus was observed by immunofluorescence. Results： Compared with control group,24 h,48h,72 h sleep deprivation significantly increased the escape latency（ P〈0. 01,n = 5）,while decreased the swimming time in the aim quadrant.Compared with the control group,the 72 h sleep deprivation significantly increased Caspase- 3 positive cells in CA1 / CA3 region of hippocampus（ P〈0. 05,n = 5）. While in 48 h sleep deprivation group,the number of Bax positive cells in CA1 / CA3 area of hippocampus increased obviously（ P〈0. 01,n = 5）. Conclusion： Sleep deprivation is an important factor affecting the cognitive function of the acute high altitude exposure rats,and its mechanism may be related to the apoptosis of hippocampus cells.

关 键 词：低压低氧 认知损害 睡眠剥夺 凋亡 

分 类 号：R740[医药卫生—神经病学与精神病学]