29例轻微型肝性脑病患者血清中特异性代谢物  

Serum metabolic profiling in 29 patients with minimal hepatic encephalopathy

作　　者：钟宇[1] 姚春[2] 姚凡[2] 黄国初[2] 王萌[2] 杨小徽[1] 

机构地区：[1]广西中医药大学,广西壮族自治区南宁市530000 [2]广西中医药大学第一附属医院肝病科,广西壮族自治区南宁市530000

出　　处：《世界华人消化杂志》2015年第34期5452-5457,共6页World Chinese Journal of Digestology

基　　金：国家自然基金资助项目;No.81160439~~

摘　　要：目的:以正常人群(normal crowd,NC)为参照,探索轻微型肝性脑病(minimal hepatic encephalopathy,MHE)患者血清中的差异性代谢物.方法:纳入于2014-02/2014-08间收集的50名NC和29例MHE患者血清样本,采用气相色谱-飞行时间质谱仪(gas chromatographytime-of-fl ight mass spectrometry,GC-TOFMS)代谢组学方法对所有样本进行检测,并采用主成份分析(principal components analysis,PCA)和偏最小二乘分析(orthogonal partial least square discriminant analysis,OPLS-DA)方法来进行建模分析,结合单维t检验进行组间比较,最终确定MHE组血清代谢谱特异性代谢物.结果:在MHE组血清中有60个与NC组血清相差异的代谢物中,上调36种,下调24种,并且MHE组与NC组的代谢物具有显著差异.上调的物质包括:同型半胱氨酸、酪氨酸、苯丙氨酸、甘氨鹅去氧胆酸、别苏氨酸、焦谷氨酸等.下调的物质包括:二十二碳六烯酸、牛磺酸、维生素E、神经酸、缬氨酸、别异亮氨酸、异亮氨酸、草酸、甘露糖、4-氨基丁酸等.结论:与NC组比,MHE组血清中存在差异性代谢物,这些代谢物可能为MHE患者的特异性生物标志物,可有助于早期诊断MHE.AIM：To screen the specific biomarkers for minimal hepatic encephalopathy（MHE）.METHODS：Serum samples were collected from 50 normal volunteers and 29 MHE patients from February 2014 to August 2014 and tested using gas chromatography-time-offlight mass spectrometry.Principal components analysis and orthogonal partial least square discriminant analysis were performed to detect the specific metabolites of MHE.RESULTS：Sixty different metabolites were found between MHE patients and normal volunteers,including homocysteine,taurine,docosahexaenoic acid,tyrosine,phenylalanine,valine,isoleucine,allo-isoleucine,etc.CONCLUSION：Compared with normal controls,different serum metabolites were found in MHE patients.These metabolites might be specific biomarkers for MHE.

关 键 词：轻微型肝性脑病 正常人群 代谢物 气相色谱-飞行时间质谱 

分 类 号：R575.3[医药卫生—消化系统]