小鼠诱导性多能干细胞向牙源性细胞分化的体内研究  

Differentiation of mouse induced pluripotent stem cells into dental epithelial cells in vivo

作　　者：刘丽[1] 宋元利 王丽英[1] 宁芳[3] 刘继延[1] 

机构地区：[1]北京军区总医院口腔科,主治医师博士北京100700 [2]总政白石桥老干部服务管理局,主治医师北京100081 [3]军械工程学院门诊部,博士河北050003

出　　处：《中华老年口腔医学杂志》2015年第6期325-328,共4页Chinese Journal of Geriatric Dentistry

基　　金：国家自然科学基金青年项目(项目编号:31400841)

摘　　要：目的:探讨小鼠诱导性多能干细胞(i PS,induced pluripotent stem cell)体内向牙源性细胞分化的潜能及适宜的诱导微环境。方法:经成釉细胞无血清条件培养液(ASF-CM)以及加入BMP4的ASF-CM(ASF-BMP4)不同微环境体外诱导的i PS细胞,分别移植入小鼠肾被膜内,H E染色及免疫组织荧光检测相关指标的表达。结果:ASF-CM组移植物HE染色可见上皮样结构生成,免疫组织荧光检测AMBN表达阳性;ASF-BMP4组可见上皮样和牙本质样结构生成,免疫组织荧光检测AMBN和DSP表达阳性。结论:ASF-BMP4提供了一个适宜的成牙微环境,能够促进i PS细胞体内形成牙源性上皮样和牙本质样结构。Objective： To study the possibility and conditions of differentiation of mouse induced pluripotent stem（i PS）cells into odontogenic cells in vivo. Methods： An in vivo transplantation assay was performed to further investigate the in vivo differentiation capacity of mouse i PS cells. Aggregates of i PS cultured in ASF-CM or in ASF-BMP4 for 14 days with CBB were implanted into the renal capsule of C57 mice. Haematoxylin and eosin（HE） and immunofluorescent staining were used to detected odontogenesis-related protein expression of AMBNand DSP. Results： ASF-CM-treated mouse i PS cells only generated ameloblast-like tissues. The expression of AMBN was found positive by immunofluorescent staining.ASF-BMP4-treated mouse i PS cells formed both ameloblast-like and odontoblast-like structures. The expression of AMBN and DSP were positive. Conclusion： These results suggest that mouse i PS cells can be induced by ASF-BMP4 to differentiate into ameloblast-like and odontoblast-like cells in vivo. ASF-BMP4 provides a suitable microenvironment for teeth regeneration.

关 键 词：诱导性多能干细胞 组织工程 微环境 成牙分化 

分 类 号：R78[医药卫生—口腔医学]