胃癌中RhoGDI2及其疑似下游靶基因14-3-3σ的原位表达和两者相关性  

Expression of RhoGDI2 and 14-3-3σ in gastric carcinoma

作　　者：石干[1] 吴杰[1] 王萍[1] 宋敏[1] 孙亮[1] 涂玉洁[1] 黄晓东[1] 

机构地区：[1]武汉市中心医院消化内科,湖北省武汉市430014

出　　处：《世界华人消化杂志》2015年第33期5282-5289,共8页World Chinese Journal of Digestology

基　　金：武汉市卫计委临床医学科研基金资助项目;No.WX15D63;武汉市中心医院院内青年科研基金资助项目;No.YQ14B04~~

摘　　要：目的:观察RhoGTP酶解离抑制蛋白2(RhoGTPase dissociation inhibitor 2,RhoGDI2)、14-3-3σ在胃癌患者组织中的表达状况,并评价其与胃癌临床病理特征的相关性,分析两者表达相关性.方法:采用免疫组织化学技术检测152例胃癌组织中RhoGDI2与14-3-3σ的表达状况,并分析两者表达相关性.结果:胃癌组织中RhoGDI2和14-3-3σ蛋白表达阳性率分别为76.97%、27.63%;胃癌中RhoGDI2和14-3-3σ的蛋白表达与下列因素密切相关:胃癌分化程度、胃癌浸润深度、淋巴结转移数、是否存在远处转移和TNM分期;胃癌组织RhoGDI2与14-3-3σ的蛋白表达水平呈负相关.结论:14-3-3σ极可能受到RhoGDI2的调控,也许是RhoGDI2在胃癌浸润转移过程中的下游靶点基因之一,两种基因很可能共同参与调控胃癌侵袭转移的过程.AIM：To detect the expression of RhoGDI2 and 14-3-3σ in gastric cancer,analyze their correlation with clinicopathological characteristics of gastric cancer,and assess their correlation.METHODS：Immunohistochemistry was used to detect the expression of RhoGDI2 and14-3-3σ in 152 gastric cancer specimens.RESULTS：The positive rates of RhoGDI2 and 14-3-3σ in gastric cancer were 76.97% and 27.63%,respectively.The expression of RhoGDI2 and 14-3-3σ was related with tumor differentiation,depth of invasion,lymph node metastasis,distant metastasis and TNM stage in gastric cancer.There was a negative correlation between the expression of RhoGDI2 and 14-3-3σ in gastric cancer.CONCLUSION：There is a correlation between the expression of RhoGDI2 and 14-3-3σ in gastric cancer.14-3-3σ is very likely regulated by RhoGDI2 and functions as a downstream target gene of RhoGDI2 in tumor invasion and metastasis.They may be both involved in regulating the invasion and metastasis of gastric cancer.

关 键 词：胃癌 RhoGDI2 14-3-3σ 临床病理特征 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]