人牙髓干细胞在不同HA含量的PLGA/HA复合支架材料上的粘附研究  

Adhesion of Human Dental Pulp Stem Cells on Poly Lactic-co-glycolic Acid/hydroxylapatite(PLGA/HA)Composite Scaffolds with Different Proportions

作　　者：王冉旭 潘爽[1] 何丽娜[1] 牛玉梅[1] 

机构地区：[1]哈尔滨医科大学口腔医院牙体牙髓科,黑龙江哈尔滨150001

出　　处：《口腔医学研究》2015年第12期1171-1173,共3页Journal of Oral Science Research

基　　金：国家自然科学基金项目(编号:81271132)

摘　　要：目的：构建不同质量构成比的PLGA/HA复合支架并评价材料机械性能;探讨不同比例PLGA/HA复合生物学支架对牙髓干细胞粘附能力的影响。方法：采用溶液浇筑/颗粒沥析技术构建出分别含有10%HA、20%HA及30%HA的PLGA/HA复合支架,对照组采用单纯PLGA支架。应用电子万能测验机检测支架的拉伸强度,扫描电镜观察表面结构。将牙髓干细胞与不同比例的PLGA/HA支架复合培养,应用DAPI染色细胞计数法检测细胞粘附能力。结果：在对3组实验组的拉伸强度测试中,10%HA组拉伸强度最高,20%组次之,两组均高于对照组,而30%HA组拉伸强度低于对照组（P〈0.05）。扫描电镜观察支架表面,显示支架孔径在100-250μm之间,孔隙间连通性良好,孔隙率较高。细胞接种于支架2、6、12h后,荧光染色显示细胞与3种支架紧密贴合,粘附良好,细胞计数结果显示各实验组细胞粘附数量均多于对照组,其中在30%HA组中细胞粘附性能最佳（P〈0.05）。结论：3种不同构成比的HA/PLGA复合支架均为良好的组织工程支架材料,其中10%HA组具有良好的机械性能,30%HA组细胞粘附性能更佳。Objective：To prepare and evaluate the Poly（lactide-co-glycolide）/Hydroxylapatite（PLGA/HA）composite scaffold with different proportions.Methods：PLGA/HA composite scaffolds with different HA contents（10%,20%and 30%）were fabricated by solvent casting/particulate leaching technology,while PLGA without HA was used as control group.The tensile strength and microstructure were detected by using electro-mechanical universal testing machines and scanning electron microscopy（SEM）.Human dental pulp stem cells were isolated,cultured and identified.The cells were seeded in the PLGA/HA scaffolds with different proportions.The adhesion properties were measured by DAPI staining.Results：The tensile strength of 10% HA was higher than that of 20%HA,and both of them showed a higher tensile strength than the control group.However,that of 30% HA was lower than control group（P〈0.05）.Scaffolds with different proportions of PLGA/HA showed high porosity and pore connectivity and the pore size was 100-250μm investigated by SEM.Cell attachment and number in PLGA/HA after the incubation of 2h,6hand 12 hwere significantly higher than the control group（P〈0.05）,and the cell adhesion ability in 30% HA group was the highest（P〈0.05）.Conclusions：The present study shows that PLGA/HA composite scaffolds with HA contents may be suitable for tissue engineering.In addition,10% HA shows a higher tensile strength,while the adhesion ability of hDPSCs in 30% HA is better.

关 键 词：PLGA HA 复合支架 人牙髓干细胞 组织工程 

分 类 号：R783.1[医药卫生—口腔医学]