牛磺酸对尼古丁致大鼠睾丸氧化损伤的保护作用  

Protective Effect of Taurine on Nicotine-Induced Oxidative Damage in Testis of Rats

作　　者：关怀[1] 左中[2] 常晓慧[3] 张晶鑫[4] 唐冬梅[4] 尚丽新[5] 

机构地区：[1]解放军第210医院妇产科,辽宁116021 [2]解放军第210医院病理科,辽宁116021 [3]解放军第210医院医务处,辽宁116021 [4]大连大学附属中山医院妇产科,辽宁116001 [5]北京军区总医院妇产科,北京100700

出　　处：《环境与职业医学》2015年第12期1162-1165,共4页Journal of Environmental and Occupational Medicine

摘　　要：[目的]探讨牛磺酸对尼古丁所致雄性大鼠睾丸氧化损伤的保护作用。[方法]32只SD雄性大鼠,随机分为阴性对照组、牛磺酸对照组、尼古丁染毒组及牛磺酸干预组。阴性对照组大鼠生理盐水1 m L皮下注射,双蒸水1 m L灌胃;牛磺酸对照组大鼠皮下注射生理盐水1 m L,灌胃牛磺酸双蒸水溶液1 m L(牛磺酸量为100 mg/kg);尼古丁染毒组大鼠皮下注射尼古丁生理盐水溶液1 m L(尼古丁量为2 mg/kg),灌胃双蒸水1 m L;牛磺酸干预组大鼠如上注射尼古丁生理盐水溶液和灌胃牛磺酸双蒸水溶液。皮下注射和灌胃每日1次,连续4周。测定大鼠睾丸系数、附睾系数、精子密度和活率;检测睾丸组织丙二醛(MDA)含量;检测睾丸组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性。[结果]睾丸系数、附睾系数、精子密度和活率:尼古丁染毒组大鼠显著低于另3组(P<0.05),牛磺酸干预组显著低于阴性对照组和牛磺酸对照组(P<0.05);睾丸组织MDA含量:尼古丁染毒组大鼠显著高于另3组(P<0.05),牛磺酸干预组显著高于阴性对照组和牛磺酸对照组(P<0.05);睾丸组织SOD、CAT、GSH-Px活性:尼古丁染毒组大鼠显著低于另3组(P<0.05),牛磺酸干预组显著低于阴性对照组和牛磺酸对照组(P<0.05)。[结论]尼古丁可致大鼠睾丸组织氧化损伤,牛磺酸对此具有抗氧化保护作用。[Objective] To assess the protective effect of taurine on nicotine-induced oxidative damage in testis of rats. [Methods] Thirty-two male SD rats were randomly divided into negative control, taurine control, nicotine exposed, and taurine intervention groups. The negative control group was treated with 1 m L physiological saline subcutaneously and 1 m L double distilled water by gavage. The taurine control group was treated with 1 m L physiological saline subcutaneously and 1 m L double distilled water containing 100 mg/kg taurine by gavage. The nicotine exposed group was treated with 1 m L physiological saline solution containing 2 mg/kg nicotine subcutaneously and 1 m L double distilled water by gavage. The taurine intervention group was treated with 1 m L physiological saline solution containing 2 mg/kg nicotine subcutaneously and 1 m L double distilled water containing 100 mg/kg taurine by gavage. All the treatments were conducted once a day and lasted four weeks. The organ coefficients of testis and epididymis, sperm count and motility rate, the content of malondialdehyde（MDA） in rat testis, and the activities of superoxide dismutase（SOD）, catalase（CAT）, and glutathione peroxidase（GSH-Px） in rat testis were examined. [Results] The organ coefficients of testis and epididymis as well as sperm count and motility rate were lower in the nicotine exposed group than in the other three groups（P〈0.05）, and were lower in the taurine intervention group than in the negative control group and taurine control group（P〈0.05）. The content of MDA in rat testis of the nicotine exposed group was significantly higher than those of the other three groups（P〈0.05）, and that of the taurine intervention group was also significantly higher than those of the negative control group and the taurine control group（P〈0.05）. The activities of SOD, CAT, and GSH-Px in rat testis were all significantly lower in the rats treated with nicotine than in the other three groups（P〈0.05）, and the taurine inte

关 键 词：牛磺酸 尼古丁 睾丸 氧化损伤 抗氧化 

分 类 号：R994.6[医药卫生—毒理学]