Expression and Significance of HIF-1α and HIF-2α in Pancreatic Cancer  

作　　者：王敏 陈美源 郭兴军 江建新 

机构地区：[1]Department of Biliary-Pancreatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology [2]Department of Hepatic-biliary-pancreatic Surgery, Hubei Cancer Hospital

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2015年第6期874-879,共6页华中科技大学学报（医学英德文版）

基　　金：supported by grants from the National Natural Science Foundation of China(No.81160311 and No.81101621);the Outstanding Young Training Project of Science and Education of Guizhou Province;China(No.[2012]177);the Postdoctoral Science Foundation of China(No.2013M531983)

摘　　要：Summary： The expression levels of hypoxia-inducible factor lalpha （HIF-lct） and HIF-2a in pancreatic cancer （PC） and their association with clinicopathologic characteristics were investigated in order to elucidate their roles in the development of PC. HIF-1 α and HIF-2α mRNA levels in 20 patients with PC were detected by quantitative real-time polymerase chain reaction. The expression of HIF-1α and HIF-2α protein in samples from other 90 patients with PC was measured by immunohistochemistry. Correlations between the expression of HIF-1 α or HIF-2α and clinicopathologica features and prognosis were analyzed. The expression of both HIF-1α and HIF-2α mRNA was up-regulated in most cancer tis- sues （P〈0.05）. HIF-1α staining was weakly positive in most cancer tissues and strongly positive in ad- jacent pancreas tissues （P〈0.05）. Clinicopathologic analysis revealed that relatively strong HIF-1α ex- pression in cancer tissues was related to greater invasion （P〈0.05）, higher tumor pathologic stage （P〈0.05）, higher American Joint Committee on Cancer （AJCC） stage （P〈0.05） and shorter overall sur- vival time （P〈0.05）. Conversely, HIF-2α staining was strongly positive in most cancer tissues and weakly positive in adjacent pancreas tissues. Clinicopathologic analysis revealed that relatively strong HIF-2α expression in cancer tissues was related to less invasion （P〈0.05）, lower tumor pathologic stage （P〈0.05）, lower AJCC stage （P〈0.05） and longer overall survival time （P〈0.05）. Moreover, the HIF-1αhigh/HIF-2αlow group showed a shorter survival time than the HIF-1αlow/HIF-2αhigh group. In con- clusion, although HIF-1α and HIF-2α mRNA expression patterns are the same, their protein expression patterns are significantly different and they play different roles in PC. Combined analysis of HIF-1α and HIF-2α expression might be useful to predict the prognosis of patients with PC.Summary： The expression levels of hypoxia-inducible factor lalpha （HIF-lct） and HIF-2a in pancreatic cancer （PC） and their association with clinicopathologic characteristics were investigated in order to elucidate their roles in the development of PC. HIF-1 α and HIF-2α mRNA levels in 20 patients with PC were detected by quantitative real-time polymerase chain reaction. The expression of HIF-1α and HIF-2α protein in samples from other 90 patients with PC was measured by immunohistochemistry. Correlations between the expression of HIF-1 α or HIF-2α and clinicopathologica features and prognosis were analyzed. The expression of both HIF-1α and HIF-2α mRNA was up-regulated in most cancer tis- sues （P〈0.05）. HIF-1α staining was weakly positive in most cancer tissues and strongly positive in ad- jacent pancreas tissues （P〈0.05）. Clinicopathologic analysis revealed that relatively strong HIF-1α ex- pression in cancer tissues was related to greater invasion （P〈0.05）, higher tumor pathologic stage （P〈0.05）, higher American Joint Committee on Cancer （AJCC） stage （P〈0.05） and shorter overall sur- vival time （P〈0.05）. Conversely, HIF-2α staining was strongly positive in most cancer tissues and weakly positive in adjacent pancreas tissues. Clinicopathologic analysis revealed that relatively strong HIF-2α expression in cancer tissues was related to less invasion （P〈0.05）, lower tumor pathologic stage （P〈0.05）, lower AJCC stage （P〈0.05） and longer overall survival time （P〈0.05）. Moreover, the HIF-1αhigh/HIF-2αlow group showed a shorter survival time than the HIF-1αlow/HIF-2αhigh group. In con- clusion, although HIF-1α and HIF-2α mRNA expression patterns are the same, their protein expression patterns are significantly different and they play different roles in PC. Combined analysis of HIF-1α and HIF-2α expression might be useful to predict the prognosis of patients with PC.

关 键 词：pancreatic cancer HIF-1Α HIF-2α AGGRESSIVENESS PROGNOSIS 

分 类 号：R735.9[医药卫生—肿瘤]