水溶性壳聚糖对美极梅奇酵母菌拮抗效力的影响  

Effect of Water-solube Chitosan on Antagonist Metschnikowia pulcherrima

作　　者：郭东起[1] 沈永娟[1] 

机构地区：[1]塔里木大学生命科学学院,南疆特色农产品深加工兵团重点实验室,新疆阿拉尔843300

出　　处：《北方园艺》2015年第24期127-130,共4页Northern Horticulture

基　　金：国家自然科学基金资助项目(31160342)

摘　　要：以冬枣为试材,采用体外及体内试验方法,研究了水溶性壳聚糖对美极梅奇酵母菌抑制冬枣采后贮藏保鲜中青霉病的效果的影响。结果表明：水溶性壳聚糖对橘青霉孢子的萌发有显著的抑制作用,壳聚糖浓度〉0.3%（w/v）,橘青霉孢子萌发状态完全受到抑制;在NYDB培养基（液体）中,壳聚糖的浓度≥0.04%（w/v）时,对酵母菌的生长有较强的抑制效果,在NYDA固体培养基中,壳聚糖浓度在0.1%～0.9%（w/v）范围内对其均有显著抑制作用;在冬枣伤口处,壳聚糖浓度≤0.1%时,对酵母菌的生长无显著影响;美极梅奇酵母菌与浓度为0.1%（w/v）壳聚糖结合处理对冬枣青霉病的抑制效果最佳。Taking winter jujubes as test material,the inhibiting effect of Metschnikowia pulcherrimaon control,blue mould of winter jujube by using water-soluble chitosan was studied by adopting the method of invitroand in vivo tests.The results showed that the water-soluble chitosan could significantly inhibit germination of Penicillium citrinumspore,when the concentration of water-soluble chitosan was more than 0.3%（w/v）,the germination of P.citrinum spore was inhibited completely.In NYDB medium,the concentration of water-soluble chitosan was 0.04%（w/v）or more,the growth of M.pulcherrima was strongly inhibited,while in NYDA（solid medium）,the growth of M.pulcherrima was significantly inhibited in the range of 0.1%-0.9%（w/v）water-soluble chitosan concentration.In the wound of winter jujube,the water-soluble chitosan concentration of 0.1% or less had no significant effect on the growth of M.pulcherrima,the combination of water-soluble chitosan concentration of 0.1% with M.pulcherrimahad the best inhibiting effect on blue mould of winter jujube.

关 键 词：美极梅奇酵母菌(Metschnikowia pulcherrima) 水溶性壳聚糖 冬枣 

分 类 号：S609.3[农业科学—园艺学]