B细胞活化因子及其特异性受体BAFF-R在B细胞性非霍奇金淋巴瘤中的表达及其意义  

Expression of BAFF and its specific receptor BAFF-R in patients with B-cell non-Hodgkin＇s lymphoma and their significance

作　　者：浦江[1] 褚少朋[1] 王梅[3] 钱晨[1] 申娴娟[2] 

机构地区：[1]南通大学附属医院临床检验诊断中心,226001 [2]南通大学附属医院外科综合实验室,226001 [3]南通大学

出　　处：《中华微生物学和免疫学杂志》2015年第11期821-826,共6页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金青年基金（81301498）

摘　　要：目的探讨B细胞活化因子（BAFF）及其特异性受体BAFF-R在B细胞性非霍奇金淋巴瘤（B-NHL）中的表达及其意义。方法ELISA及荧光定量-聚合酶链反应（RTQ-PCR）测定临床标本BAFF及其特异性受体BAFF-R蛋白与mRNA的表达水平；RT—PCR、Western blot、荧光免疫细胞化学法和激光共聚焦技术鉴定人Burkitt’s 淋巴瘤细胞Raji中BAFF及BAFF-R的表达与定位；运用免疫组化的方法分析B—NHL患者肿瘤组织中BAFF的表达；WST细胞增殖试验及TUNEL法检测BAFF及其受体BAFF-R对B—NHLRaji细胞生长、存活与凋亡的影响。直线相关分析LDH浓度与BAFF蛋白及mRNA表达水平的关系。结果（1）B-NHL患者BAFF蛋白或mRNA表达水平显著高于正常对照组（P〈0．01）；（2）Raji细胞表达BAFF及其受体BAFF-R，并定位表达于Raji细胞的质膜上；（3）BAFF在B—NHL患者的表达量与B—NHL组织亚型有关，BAFF在小B细胞恶性淋巴瘤、弥漫性大B细胞淋巴瘤、黏膜相关淋巴瘤和滤泡性淋巴瘤中表达量较高，而在套细胞淋巴瘤中表达量较低。（4）BAFF促进Raji细胞的生长与存活；（5）直线相关分析显示乳酸脱氢酶（LDH）与BAFF蛋白及mRNA表达水平呈显著相关性。结论BAFF及其特异性受体BAFF-R对于B—NHL的发生、发展起着重要的作用。Objective To investigate the expression of B-cell activating factor （BAFF） and its specific receptor BAFF-R in patients with B-cell non-Hodgkin＇s lymphoma （B-NHL） and to analyze the correlations between BAFF and the development of B-NHL. Methods RTQ-PCR and Western blot assay were used to measure the expression of BAFF and its specific receptor BAFF-R in patients with B-NHL. Fluorescence immunocytochemical staining was used to determine the localization of BAFF and BAFF-R in Raji cells, a B-NHL cell line. The expression of BAFF in tumor tissues from patients with B-NHL of different histologic subtypes was measured by immunohistochemistry. WST proliferation and TUNEL assays were used to evaluate the effects of BAFF and BAFF-R on the proliferation, survival rate and apoptosis of Raji cells. Linear correlations between the concentrations of lactate dehydrogenase （LDH） and the expression of BAFF and BAFF at mRNA and protein levels in patients with B-NHL were analyzed. Results BAFF and its specific receptor BAFF-R were expressed in Raji cells and played an important role in the survival and proliferation of B-NHL cell line. The expression of BAFF in tumor cells from patients with B-NHL varied with the different histologic subtypes of B-NHL. Patients with small B-cell malignant lymphoma, large B-cell lymphoma （ LBCL）, mucosa-associated lymphoid tissue lymphoma （ MALT lymphoma） and follicular lymphoma showed higher levels of BAFF, while those with mantle cell lymphoma showed lower levels of BAFF. Compared with the healthy subjects, patients with B-NHL showed significantly increased expression of BAFF at mRNA and protein levels. The levels of LDH were closely related to the expression of BAFF at mRNA and protein levels. Conclusion BAFF and its specific receptor BAFF-R might play an important role in the growth andsurvival of malignant B cells.

关 键 词：B细胞性非霍奇金淋巴瘤 B细胞活化因子 B细胞活化因子受体 乳酸脱氢酶 

分 类 号：R733.1[医药卫生—肿瘤]