小鼠骨髓间充质干细胞SCA-1^+/CD45^+/CD31^+亚群向心肌样细胞分化的分子基础  

Molecular basis of mouse bone marrow mesenchymal stem cells subsets SCA-1^+/CD45^+/CD31^+ differentation to cardiomyocyte-like cells

作　　者：贺继刚[1] 李洪荣[1] 桂龙升[1] 李永武[1] 严丹[1] 王平[1] 

机构地区：[1]云南省第一人民医院心脏大血管外科,昆明650032

出　　处：《临床心血管病杂志》2015年第12期1332-1335,共4页Journal of Clinical Cardiology

基　　金：国家自然科学基金(No:81460073);云南省科技厅-昆明医科大学应用基础研究联合专项资助(No:2014FB089)

摘　　要：目的:探讨小鼠骨髓间充质干细胞(mBMSCs)亚群SCA-1^+/CD45^+/CD31^+向心肌样细胞分化的分子基础。方法:以小鼠心脏干细胞表面分化抗原检测mBMSCs后以CD45、CD31为标准分选得到4个亚群。备亚群和心肌细胞共培养后检测α-actin、Cx43、Desmin、cTnI的表达。并采用基因芯片完成Agilent小鼠全基因4*44K芯片从分子水平对备亚群和心肌细胞共培养结果进行探讨。结果:SCA-1^+/CD45^+/CD31^+亚群和心肌细胞共培养后更易表达出心肌细胞标志性分子。通过Agilent小鼠全基因4*44K芯片将SCA-1^+/CD45^+/CD31^+与其他组的心血管发育基因的聚类分析及Network结果共同比较可见两者无交集,以差异基因均为4倍表达为标准,因而以Network结果为主。可见:SETD2、NCL、EPOR、Rock2为感兴趣基因。结论:mBMSCs为多克隆的细胞群体。SCA-1^+/CD45^+/CD31^+在向心肌定向分化及改善心功能方面优于其他亚群,其分子机制在于SETD2、NCL、EPOR、Rock2基因表达较其他亚组多见。Objective：To investigate molecular basis of the mouse bone marrow mesenchymal stem cells（mBMSCs） subsets SCA-1^＋ /CD45^＋ /CD31^＋ cell differentiation into cardiomyocytes.Method：Mouse cardiac stem cell surface differentiation antigen was used to detect mBMSCs,then mBMSCs was sorted into four subgroups according to CD45,CD31 standard.Subsets and myocardial cells were co-cultured,and the the expression of cractin,Cx43,Desmin,cTnI was detected.Agilent gene chip was used to detect mouse genome from the molecular level.Result：SCA-1^＋ /CD45^＋ /CD31^＋ subpopulation expressed more iconic molecules of myocardial cells after cocultured with cardiomyocytes.By Agilent 4 ＊ 44 K chip detection,cardiovascular development genes of SCA-1^＋/CD45^＋/CD31^＋ and other subgroups cluster analysis and Network analysis showed that there was no intersection of differences gene expression with other subgroups.The results of Network was more important when adopted 4times gene expression as the standard,thus SETD2,NCL,EPOR,Rock2 were interest genes.Conclusion：mBMSCs are the polyclonal population of cells.The improvement effects of SCA-1^＋ /CD45^＋ /CD31^＋ differentiation into cardiomyocytes on heart function are better than those of other subgroups,the molecular mechanism involved in more expression of SETD2,NCL,EPOR and in SCA-1^＋/CD45^＋/CD31^＋.

关 键 词：小鼠骨髓间充质干细胞 亚群 基因 

分 类 号：R394.3[医药卫生—医学遗传学]