宫颈病变组织中P53、Ki-67、CD34的表达及临床意义  

Expressions and Clinical Significances of P53, Ki-67 and CD34 in the Cervical Lesion

作　　者：冯仙明[1] 庞燕[1] 梁锦辉[1] 

机构地区：[1]广东省农垦中心医院湛江肿瘤医院,广东湛江524002

出　　处：《肿瘤药学》2015年第6期449-453,共5页Anti-Tumor Pharmacy

摘　　要：目的探讨宫颈病变组织中P53、Ki-67、CD34的表达及其临床意义。方法应用免疫组化SP法测定正常宫颈上皮、宫颈上皮内瘤变和宫颈浸润癌组织中P53、Ki-67、CD34的表达。结果 P53在正常宫颈上皮、宫颈上皮内瘤变和宫颈浸润癌组织中的阳性表达率分别为0%(0/28)、39.58%(19/48)和69.44%(25/36),Ki-67的阳性率分别为3.57%(1/28)、52.08%(25/48)和86.11%(31/36),CD34标记的MVD值分别为(12.46±4.63)/HP、(30.12±7.46)/HP和(42.12±5.48)/HP,P53、Ki-67、CD34在三种宫颈病变中的阳性表达率两两比较,差异均有统计学意义。结论 P53、Ki-67、CD34表达强度均随宫颈病变的进展而增加,P53和Ki-67的表达与浸润癌的分化程度无关,CD34的表达与浸润癌的分化程度正相关。检测P53、Ki-67和CD34的表达对CIN癌变的早期诊断及其分层治疗有一定的指导意义。Objective To investigate the expressions and clinical significances of p53, ki-67 and cd34 in the cervical lesions. Methods Immunohistochemical method was used to detect the expressions of P53, Ki-67 and CD34 in the normal uterine cervix, cervical intraepithelial neoplasia（CIN） and cervical infiltrating carcinoma. Results In normal uterine cervix, CIN and cervical in- filtrating carcinoma, the positive expression rates of P53 were respectively 0%（0/28）, 39.58% （19/48） and 69.44% （25/36）, and the positive expression rates of Ki-67 were respectively 3.57%（1/28）, 52.08% （25/48） and 86.11%（31/36）. The microvessel density （MVD） of marked CD34 was respectively （12.46±4.63）/HP, （30.12±7.46）/HP and （42.12±5.48）/HP in the above mentioned tissues. The pair-wise comparison showed that the positive expression rates of P53, Ki-67 and CD34 had statistical difference in the CIN I-II, CIN III and cervical infiltrating carcinoma tissues（P〈0.05）. Conclusion The expressions of P53, Ki-67 and CD34 increased with the progression of the cervical lesions. The expression of P53 and Ki-67 had no correlation with the differentiation degree of infiltrating carcinoma, while the expression of CD34 was positively correlated with the differentiation degree of cervical infiltrating carcinoma. Combined detection of P53, Ki-67 and CD34 can be helpful for the early diagnosis and classification of CIN.

关 键 词：宫颈病变 P53 KI-67 CD34 微血管密度 

分 类 号：R737.33[医药卫生—肿瘤]